Literature summary for 1.18.1.1 extracted from

Nishikawa, K.; Shomura, Y.; Kawasaki, S.; Niimura, Y.; Higuchi, Y.
Crystal structure of NADH:rubredoxin oxidoreductase from Clostridium acetobutylicum: A key component of the dioxygen scavenging system in obligatory anaerobes (2010), Proteins, 78, 1066-1070.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged enzyme, sitting-drop vapour-diffusion method, 20°C, 0.001 l of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.0, is mixed with 0.001 ml of reservoir solution containing 35% v/v PEG 400, 0.1 M Tris-HCl, pH 8.5, and 0.15 M MgCl2, and equilibrated against 0.1 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.1 A resolution, structure modelling, overview	Clostridium acetobutylicum

Crystallization (Comment)

Organism

purified recombinant His6-tagged enzyme, sitting-drop vapour-diffusion method, 20°C, 0.001 l of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.0, is mixed with 0.001 ml of reservoir solution containing 35% v/v PEG 400, 0.1 M Tris-HCl, pH 8.5, and 0.15 M MgCl2, and equilibrated against 0.1 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.1 A resolution, structure modelling, overview

Clostridium acetobutylicum

Organism

Organism	UniProt	Comment	Textmining
Clostridium acetobutylicum	Q9AL95	-	-

Subunits

Subunits	Comment	Organism
monomer	NROR exists as a monomer in solution, the overall structure of NROR displays a GR-fold	Clostridium acetobutylicum

Synonyms

Synonyms	Comment	Organism
NADH:rubredoxin oxidoreductase	-	Clostridium acetobutylicum
NROR	-	Clostridium acetobutylicum

Cofactor

Cofactor	Comment	Organism	Structure
FAD	binding structure, overview	Clostridium acetobutylicum
NADH	binding structure, overview	Clostridium acetobutylicum

General Information

General Information	Comment	Organism
additional information	the enzyme's disulfide bonds are not involved in the electron transfer from NADH to rubredoxin catalyzed by NROR	Clostridium acetobutylicum

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Release 2023.1 (January 2023)