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Literature summary for 1.18.1.2 extracted from
- Functional plasticity and catalytic efficiency in plant and bacterial ferredoxin-NADP(H) reductases (2004), Biochim. Biophys. Acta, 1698, 155-165.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | forward and reverse reactions follow different kinetic mechanism, overview | Cyanophora paradoxa | |
| additional information | - |
additional information | forward and reverse reactions follow different kinetic mechanism, overview | Chlamydomonas reinhardtii | |
| additional information | - |
additional information | forward and reverse reactions follow different kinetic mechanism, overview | Escherichia coli | |
| additional information | - |
additional information | forward and reverse reactions follow different kinetic mechanism, overview | Spinacia oleracea | |
| additional information | - |
additional information | forward and reverse reactions follow different kinetic mechanism, overview | Rhodobacter capsulatus | |
| additional information | - |
additional information | forward and reverse reactions follow different kinetic mechanism, overview | Azotobacter vinelandii | |
| additional information | - |
additional information | forward and reverse reactions follow different kinetic mechanism, overview | Anabaena sp. | |
| additional information | - |
additional information | forward and reverse reactions follow different kinetic mechanism, overview | Leptospira interrogans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| additional information | nucleus-encoded enzyme | Cyanophora paradoxa | - |
- |
| plastid | - |
Spinacia oleracea | 9536 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | enzyme is involved in anaerobic metabolism, phylogenetic evolution, relationships, and classification, overview | ? | - |
? |  |
| additional information | Azotobacter vinelandii | enzyme is involved in nitrogenase reduction, phylogenetic evolution, relationships, and classification, overview | ? | - |
? | |
| additional information | Spinacia oleracea | enzyme is involved in photosynthesis and nitrite assimilation, phylogenetic evolution, relatiionships, and classification, overview | ? | - |
? | |
| additional information | Chlamydomonas reinhardtii | phylogenetic evolution, relatiionships, and classification, overview | ? | - |
? | |
| additional information | Rhodobacter capsulatus | phylogenetic evolution, relatiionships, and classification, overview | ? | - |
? | |
| additional information | Leptospira interrogans | phylogenetic evolution, relatiionships, and classification, overview | ? | - |
? | |
| additional information | Cyanophora paradoxa | phylogenetic evolution, relationships, and classification, overview | ? | - |
? | |
| additional information | Anabaena sp. | phylogenetic evolution, relationships, and classification, overview | ? | - |
? | |
| additional information | Anabaena sp. PCC7119 | phylogenetic evolution, relationships, and classification, overview | ? | - |
? | |
| reduced ferredoxin + NADP+ | Cyanophora paradoxa | - |
oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | Chlamydomonas reinhardtii | - |
oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | Escherichia coli | - |
oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | Spinacia oleracea | - |
oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | Rhodobacter capsulatus | - |
oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | Azotobacter vinelandii | - |
oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | Anabaena sp. | - |
oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | Leptospira interrogans | - |
oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | Anabaena sp. PCC7119 | - |
oxidized ferredoxin + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena sp. | - |
PCC7119 | - |
| Anabaena sp. PCC7119 | - |
PCC7119 | - |
| Azotobacter vinelandii | - |
- |
- |
| Chlamydomonas reinhardtii | - |
- |
- |
| Cyanophora paradoxa | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Leptospira interrogans | - |
parasitic bacterium | - |
| Rhodobacter capsulatus | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism of forward and reverse reactions | Cyanophora paradoxa | |
| 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism of forward and reverse reactions | Chlamydomonas reinhardtii | |
| 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism of forward and reverse reactions | Escherichia coli | |
| 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism of forward and reverse reactions | Spinacia oleracea | |
| 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism of forward and reverse reactions | Rhodobacter capsulatus | |
| 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism of forward and reverse reactions | Azotobacter vinelandii | |
| 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism of forward and reverse reactions | Anabaena sp. | |
| 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism of forward and reverse reactions | Leptospira interrogans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme is involved in anaerobic metabolism, phylogenetic evolution, relationships, and classification, overview | Escherichia coli | ? | - |
? | |
| additional information | enzyme is involved in nitrogenase reduction, phylogenetic evolution, relationships, and classification, overview | Azotobacter vinelandii | ? | - |
? | |
| additional information | enzyme is involved in photosynthesis and nitrite assimilation, phylogenetic evolution, relatiionships, and classification, overview | Spinacia oleracea | ? | - |
? | |
| additional information | phylogenetic evolution, relatiionships, and classification, overview | Chlamydomonas reinhardtii | ? | - |
? | |
| additional information | phylogenetic evolution, relatiionships, and classification, overview | Rhodobacter capsulatus | ? | - |
? | |
| additional information | phylogenetic evolution, relatiionships, and classification, overview | Leptospira interrogans | ? | - |
? | |
| additional information | phylogenetic evolution, relationships, and classification, overview | Cyanophora paradoxa | ? | - |
? | |
| additional information | phylogenetic evolution, relationships, and classification, overview | Anabaena sp. | ? | - |
? | |
| additional information | phylogenetic evolution, relationships, and classification, overview | Anabaena sp. PCC7119 | ? | - |
? | |
| NADPH + acceptor | diaphorase activity, acceptors can be complexed metals or aromatic molecules | Anabaena sp. | NADP+ + reduced acceptor | - |
? | |
| NADPH + acceptor | diaphorase activity, acceptors can be complexed metals or aromatic molecules | Anabaena sp. PCC7119 | NADP+ + reduced acceptor | - |
? | |
| reduced ferredoxin + NADP+ | - |
Cyanophora paradoxa | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Chlamydomonas reinhardtii | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Escherichia coli | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Spinacia oleracea | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Rhodobacter capsulatus | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Azotobacter vinelandii | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Anabaena sp. | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Leptospira interrogans | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | association of ferredoxin with the enzyme is steered by electrostatic interactions | Cyanophora paradoxa | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | association of ferredoxin with the enzyme is steered by electrostatic interactions | Chlamydomonas reinhardtii | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | association of ferredoxin with the enzyme is steered by electrostatic interactions | Escherichia coli | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | association of ferredoxin with the enzyme is steered by electrostatic interactions | Rhodobacter capsulatus | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | association of ferredoxin with the enzyme is steered by electrostatic interactions | Azotobacter vinelandii | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | association of ferredoxin with the enzyme is steered by electrostatic interactions | Anabaena sp. | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | association of ferredoxin with the enzyme is steered by electrostatic interactions | Leptospira interrogans | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | ferredoxin contains a [2Fe2S] cluster and binds to the concave surface of the FAD domain, association of ferredoxin with the enzyme is steered by electrostatic interactions | Spinacia oleracea | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Anabaena sp. PCC7119 | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | association of ferredoxin with the enzyme is steered by electrostatic interactions | Anabaena sp. PCC7119 | oxidized ferredoxin + NADPH | - |
r | |
| reduced ferredoxin + NADP+ | - |
Chlamydomonas reinhardtii | oxidized ferredoxin + NADPH + H+ | - |
r | |
| reduced ferredoxin + NADP+ | - |
Escherichia coli | oxidized ferredoxin + NADPH + H+ | - |
r | |
| reduced ferredoxin + NADP+ | - |
Spinacia oleracea | oxidized ferredoxin + NADPH + H+ | - |
r | |
| reduced ferredoxin + NADP+ | - |
Azotobacter vinelandii | oxidized ferredoxin + NADPH + H+ | - |
r | |
| reduced ferredoxin + NADP+ | - |
Anabaena sp. | oxidized ferredoxin + NADPH + H+ | - |
r | |
| reduced ferredoxin + NADP+ | - |
Anabaena sp. PCC7119 | oxidized ferredoxin + NADPH + H+ | - |
r | |
| reduced flavodoxin + NADP+ | - |
Escherichia coli | oxidized flavodoxin + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | 2-domain structure | Cyanophora paradoxa |
| More | 2-domain structure | Chlamydomonas reinhardtii |
| More | 2-domain structure | Rhodobacter capsulatus |
| More | 2-domain structure | Leptospira interrogans |
| More | 2-domain structure, structure analysis | Escherichia coli |
| More | 2-domain structure, structure analysis | Spinacia oleracea |
| More | 2-domain structure, structure analysis | Azotobacter vinelandii |
| More | 2-domain structure, structure analysis | Anabaena sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Cyanophora paradoxa |
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Chlamydomonas reinhardtii |
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Escherichia coli |
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Spinacia oleracea |
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Rhodobacter capsulatus |
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Azotobacter vinelandii |
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Anabaena sp. |
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Leptospira interrogans |
| ferredoxin-NADP(H) reductase | - |
Cyanophora paradoxa |
| ferredoxin-NADP(H) reductase | - |
Chlamydomonas reinhardtii |
| ferredoxin-NADP(H) reductase | - |
Escherichia coli |
| ferredoxin-NADP(H) reductase | - |
Spinacia oleracea |
| ferredoxin-NADP(H) reductase | - |
Rhodobacter capsulatus |
| ferredoxin-NADP(H) reductase | - |
Azotobacter vinelandii |
| ferredoxin-NADP(H) reductase | - |
Anabaena sp. |
| ferredoxin-NADP(H) reductase | - |
Leptospira interrogans |
| FNR | - |
Cyanophora paradoxa |
| FNR | - |
Chlamydomonas reinhardtii |
| FNR | - |
Escherichia coli |
| FNR | - |
Spinacia oleracea |
| FNR | - |
Rhodobacter capsulatus |
| FNR | - |
Azotobacter vinelandii |
| FNR | - |
Anabaena sp. |
| FNR | - |
Leptospira interrogans |
| More | enzyme belongs more to the plant-type enzyme family than to the bacterial-type enzyme family | Cyanophora paradoxa |
| More | enzyme belongs to the bacterial-type enzyme family | Escherichia coli |
| More | enzyme belongs to the bacterial-type enzyme family | Azotobacter vinelandii |
| More | enzyme belongs to the plant-type enzyme family | Chlamydomonas reinhardtii |
| More | enzyme belongs to the plant-type enzyme family | Spinacia oleracea |
| More | enzyme belongs to the plant-type enzyme family | Rhodobacter capsulatus |
| More | enzyme belongs to the plant-type enzyme family | Anabaena sp. |
| More | enzyme belongs to the plant-type enzyme family, result of lateral gene transfer | Leptospira interrogans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | low efficiency | Rhodobacter capsulatus | |
| additional information | - |
additional information | low efficiency | Azotobacter vinelandii | |
| additional information | - |
additional information | the low efficiency is intrinsic to the reductase itself | Escherichia coli | |
| 0.004 | - |
reduced flavodoxin | flavodoxin I or II | Escherichia coli | |
| 0.15 | - |
reduced ferredoxin | - |
Escherichia coli | |
| 1 | - |
reduced ferredoxin | - |
Azotobacter vinelandii | |
| 90 | 174 | reduced ferredoxin | - |
Chlamydomonas reinhardtii | |
| 200 | 600 | reduced ferredoxin | - |
Spinacia oleracea | |
| 200 | 600 | reduced ferredoxin | - |
Anabaena sp. | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | extended conformation | Cyanophora paradoxa | |
| FAD | extended conformation | Chlamydomonas reinhardtii | |
| FAD | extended conformation | Rhodobacter capsulatus | |
| FAD | extended conformation | Leptospira interrogans | |
| FAD | extended conformation, interaction via Tyr96, direct electron transfer between FAD and ferredoxin [2Fe2S] center, modeling of conformation of C4alpha polypeptide backbone and FAD | Spinacia oleracea | |
| FAD | extended conformation, modeling of conformation of C4alpha polypeptide backbone and FAD, binding domain is N-terminal | Anabaena sp. | |
| FAD | folded conformation, modeling of conformation of C4alpha polypeptide backbone and FAD, binding domain is N-terminal | Escherichia coli | |
| FAD | folded conformation, modeling of conformation of C4alpha polypeptide backbone and FAD, binding domain is N-terminal | Azotobacter vinelandii | |
| additional information | poor activity with NAD(H) | Anabaena sp. | |
| NADP+ | - |
Cyanophora paradoxa | |
| NADP+ | - |
Chlamydomonas reinhardtii | |
| NADP+ | - |
Rhodobacter capsulatus | |
| NADP+ | - |
Leptospira interrogans | |
| NADP+ | C-terminal binding domain | Escherichia coli | |
| NADP+ | C-terminal binding domain | Azotobacter vinelandii | |
| NADP+ | C-terminal binding domain | Anabaena sp. | |
| NADP+ | interaction via Tyr314, binding site at the C-terminus | Spinacia oleracea | |
| NADPH | - |
Cyanophora paradoxa | |
| NADPH | - |
Chlamydomonas reinhardtii | |
| NADPH | - |
Rhodobacter capsulatus | |
| NADPH | - |
Leptospira interrogans | |
| NADPH | C-terminal binding domain | Escherichia coli | |
| NADPH | C-terminal binding domain | Azotobacter vinelandii | |
| NADPH | C-terminal binding domain | Anabaena sp. | |
| NADPH | interaction via Tyr314, binding site at the C-terminus | Spinacia oleracea | |
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