Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.18.1.2 extracted from

  • Nakajima, M.; Sakamoto, T.; Wada, K.
    The complete purification and characterization of three forms of ferredoxin-NADP+ oxidoreductase from a thermophilic cyanobacterium Synechococcus elongatus (2002), Plant Cell Physiol., 43, 484-493.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene petH, DNA and amino acid sequence determination and analysis Synechococcus elongatus

General Stability

General Stability Organism
complex formation with phycocyanin via the CpcD-like domain stabilizes the enzyme Synechococcus elongatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus
43415
-
x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus
45000
-
x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus
78000
-
x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus Q93RE3 i.e. Thermosynechococcus elongatus, single copy gene petH
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification dissociation of the enzyme-phycocyanin complex induces the specific proteolysis between the CpcD-like domain and the FAD-binding domain to give rise to the 34 kDa enzyme form Synechococcus elongatus

Purification (Commentary)

Purification (Comment) Organism
34 kDa enzyme form, 78 kDa complexed enzyme by cation exchange chromatography, and gel filtration, active 45 kDa enzyme form to homogeneity by ammonium sulfate precipitation, DEAE ion exchange chromatography, and gel filtration Synechococcus elongatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Synechococcus elongatus
11
-
purified 45 kDa enzyme form, substrate 2,6-dichlorophenolindophenol, 22°C Synechococcus elongatus

Storage Stability

Storage Stability Organism
-30°C, aggregation of the 45 kDa enzyme form occurs during storage, 50% loss of activity after 1 week Synechococcus elongatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase activity Synechococcus elongatus NADP+ + reduced 2,6-dichlorophenolindophenol
-
?
reduced ferredoxin + NADP+
-
Synechococcus elongatus oxidized ferredoxin + NADPH
-
?

Subunits

Subunits Comment Organism
? x * 43415, amino acid sequence calculation, x * 45000, active enzyme form containing the CpcD-like domain, SDS-PAGE, x * 34000, enzyme form lacking the CpcD-like domain, SDS-PAGE, x * 78000, enzyme in complex with phycocyanin, SDS-PAGE Synechococcus elongatus

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ oxidoreductase
-
Synechococcus elongatus
FNR
-
Synechococcus elongatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
temperature dependence, overview Synechococcus elongatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 activity at 52°C is 3fold higher than activity at 25°C Synechococcus elongatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
5 min, completely stable Synechococcus elongatus
80
-
5 min, loss of 60% activity Synechococcus elongatus
85
-
5 min, inactivation Synechococcus elongatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Synechococcus elongatus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Synechococcus elongatus
NADP+
-
Synechococcus elongatus