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Literature summary for 1.18.1.2 extracted from

  • Pandini, V.; Caprini, G.; Tedeschi, G.; Seeber, F.; Zanetti, G.; Aliverti, A.
    Roles of the species-specific subdomain and the N-terminal peptide of Toxoplasma gondii ferredoxin-NADP+ reductase in ferredoxin binding (2006), Biochemistry, 45, 3563-3571.
    View publication on PubMed
Search for more literature for 1.18.1.2

Protein Variants

Protein Variants Comment Organism
DELTA1-15 removal of the N-terminal 15 residues of enzyme enhances the interaction with ferredoxin Toxoplasma gondii
DELTA81-118 deleted amino acid comprise a species-specific subdomain containing an insertion of 28 residues, deletion results in an catalytically active enzyme form with 10fold decreased affinity for ferredoxin Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 reduced ferredoxin mutant lacking amino acids 81 to 118, pH 8.2, 25°C Toxoplasma gondii
0.015
-
 NADPH wild-type, pH 9.0, 25°C Toxoplasma gondii
0.016
-
 NADPH mutant lacking amino acids 81 to 118, pH 9.0, 25°C Toxoplasma gondii
0.018
-
 reduced ferredoxin wild-type, pH 8.2, 25°C Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 Toxoplasma gondii
37600
-
 gel filtration, mutant lacking amino acids 81 to 118 Toxoplasma gondii
39000
-
 1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 Toxoplasma gondii
46300
-
 gel filtration, wildtype Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxidized ferredoxin + NADPH
-
 Toxoplasma gondii reduced ferredoxin + NADP+
-
 ?
reduced ferredoxin + NADP+
-
 Toxoplasma gondii oxidized ferredoxin + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 Toxoplasma gondii
More study on enzyme conformation by limited proteolysis. Protein substrate ferredoxin protects ferredoxin-NADP+ reductase against cleavage both at its N-terminal peptide and at its largest sequence insertion of 28 residues Toxoplasma gondii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
130
-
 reduced ferredoxin mutant lacking pH 8.2, 25°C Toxoplasma gondii
260
-
 reduced ferredoxin wild-type, pH 8.2, 25°C Toxoplasma gondii
424
-
 NADPH mutant lacking pH 9.0, 25°C Toxoplasma gondii
540
-
 NADPH wild-type, pH 9.0, 25°C Toxoplasma gondii