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Literature summary for 1.18.1.2 extracted from

  • Alte, F.; Stengel, A.; Benz, J.P.; Petersen, E.; Soll, J.; Groll, M.; Boelter, B.
    Ferredoxin:NADPH oxidoreductase is recruited to thylakoids by binding to a polyproline type II helix in a pH-dependent manner (2010), Proc. Natl. Acad. Sci. USA, 107, 19260-19265.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.18.1.2

Crystallization (Commentary)

Crystallization (Comment) Organism
analysis of the enzyme in complex with a synthetic peptide representing the first FNR-binding repeat from Pisum sativum Tic62, including the strictly conserved core motif KTEQPLSPYTAYDDLKPPSSPSPTKPS, based on the crystal structure PDB ID 1QG0, X-ray diffraction structure analysis at 1.7 A resolution, molecular replacement Pisum sativum

General Stability

General Stability Organism
interaction with Tic62 stabilizes FNR Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast FNR is a soluble protein associated with thylakoid membranes Pisum sativum 9507
-
thylakoid membrane FNR is a soluble protein associated with thylakoid membranes Pisum sativum 42651
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 reduced ferredoxin + NADP+ Pisum sativum
-
 2 oxidized ferredoxin + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin + NADP+
-
 Pisum sativum 2 oxidized ferredoxin + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
More FNR-membrane recruitment motif, FNR-MRM, forms a polyproline helix that interacts with a proline recognition domain on FNR. FNR displays a pH-dependent high affinity to the MRM, loop residues contribute to multiple interactions with both FNR subunits Pisum sativum

Synonyms

Synonyms Comment Organism
ferredoxin:NADPH oxidoreductase
-
 Pisum sativum
FNR
-
 Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 assay at Pisum sativum

General Information

General Information Comment Organism
additional information FNR interacts with Tic62, a member of the Tic complex, i.e. translocon at the inner envelope of chloroplasts, involved in redox-regulation of protein import into chloroplasts. Tic62 represents a major FNR interaction protein partner at the thylakoids, and binding to Tic62 clearly increases the stability of FNR. The specific interaction with FNR is mediated by a conserved sequence motif rich in proline and serine residues, located in the C terminus of Tic62 Pisum sativum