Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Pseudomonas putida | |
2,2'-dipyridyl | - |
Pseudomonas putida | |
PCMB | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
ferricytochrome c | - |
Pseudomonas putida | |
0.0105 | - |
NADH | reaction with cytochrome c | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 46000, SDS-PAGE | Pseudomonas putida |
46500 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
NAD-ferredocinTOL reductase component of toluene dioxygenase | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.3 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, 3 months, little loss of activity | Pseudomonas putida |
0-4°C, stable for up to 30 h | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Pseudomonas putida | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricytochrome c + NADH | - |
Pseudomonas putida | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
ferricytochrome c + NADH | - |
Pseudomonas putida | ferrocytochrome c + NAD+ + H+ | - |
r | |
NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Pseudomonas putida | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
NADH + oxidized nitro blue tetrazolium + H+ | - |
Pseudomonas putida | NAD+ + reduced nitro blue tetrazolium | - |
? | |
oxidized ferredoxin + NADH | - |
Pseudomonas putida | reduced ferredoxin + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
NAD-ferredocinTOL reductase | component of toluene dioxygenase | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7.5 | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | can bind one mol of FAD per mol of enzyme, Km: 2.5 nM | Pseudomonas putida | |
NADH | - |
Pseudomonas putida |