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Literature summary for 1.18.1.4 extracted from

  • Ma, K.; Adams, M.W.
    A hyperactive NAD(P)H:rubredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus (1999), J. Bacteriol., 181, 5530-5533.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
FAD the enzyme contains one flavin adenine dinucleotide (FAD) molecule per mol Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
NADPH pH 8.0, 80°C, cosubstrate benzyl viologen Pyrococcus furiosus
0.01
-
oxidized rubredoxin pH 8.0, 80°C, cosubstrate NADPH Pyrococcus furiosus
0.034
-
NADH pH 8.0, 80°C, cosubstrate benzyl viologen Pyrococcus furiosus
0.05
-
oxidized rubredoxin pH 8.0, 80°C, cosubstrate: NADPH Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metals can be detected Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 45000, SDS-PAGE Pyrococcus furiosus
51000
-
gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 oxidized rubredoxin + NADH Pyrococcus furiosus
-
2 reduced rubredoxin + NAD+ + H+
-
?
2 oxidized rubredoxin + NADPH Pyrococcus furiosus rubredoxin is the most efficient electron acceptor 2 reduced rubredoxin + NADP+ + H+
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20000
-
pH 8.0, 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 oxidized rubredoxin + NADH
-
Pyrococcus furiosus 2 reduced rubredoxin + NAD+ + H+
-
?
2 oxidized rubredoxin + NADPH rubredoxin is the most efficient electron acceptor Pyrococcus furiosus 2 reduced rubredoxin + NADP+ + H+
-
?
additional information the enzyme does not reduce Pyrococcus furiosus ferredoxin Pyrococcus furiosus ?
-
?
oxidized benzyl viologen + NADH
-
Pyrococcus furiosus reduced benzyl viologen + NAD+
-
?
oxidized benzyl viologen + NADPH
-
Pyrococcus furiosus reduced benzyl viologen + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
NROR
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 80 about 70fold increase in kcat/Km for oxidized rebredoxin increases from 25°C to 80°C Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
12 h, 50% loss of activity Pyrococcus furiosus
95
-
2 h, 50% loss of activity Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
221
-
oxidized rubredoxin pH 8.0, 25°C, cosubstrate NADPH Pyrococcus furiosus
341
-
NADPH pH 8.0, 80°C, cosubstrate: benzyl viologen Pyrococcus furiosus
354
-
NADH pH 8.0, 80°C, cosubstrate: benzyl viologen Pyrococcus furiosus
15000
-
oxidized rubredoxin pH 8.0, 80°C, cosubstrate NADPH Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10400
-
NADH pH 8.0, 80°C, cosubstrate benzyl viologen Pyrococcus furiosus
22050
-
oxidized rubredoxin pH 8.0, 80°C, cosubstrate NADPH Pyrococcus furiosus
68300
-
NADPH pH 8.0, 80°C, cosubstrate benzyl viologen Pyrococcus furiosus
300000
-
oxidized rubredoxin pH 8.0, 80°C, cosubstrate NADPH Pyrococcus furiosus