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Literature summary for 1.18.1.7 extracted from

  • Haigler, B.E.; Gibson, D.T.
    Purification and properties of NADH-ferredoxinNAP reductase, a component of naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816 (1990), J. Bacteriol., 172, 457-464.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.18.1.7

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate 50% inhibition at 10 mM Pseudomonas putida
N-ethylmaleimide 67% inhibition at 10 mM Pseudomonas putida
o-phenanthroline 63% inhibition at 10 mM Pseudomonas putida
p-chloromercuribenzoate 94% inhibition at 0.0005 mM Pseudomonas putida
Sodium azide 46% inhibition at 40 mM Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the purified protein contains 1.8 g atoms of iron Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000, SDS-PAGE Pseudomonas putida
37000
-
 gel filtration Pseudomonas putida
37104
-
 1 * 37104, calculated from amino acid sequence Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q52126
-
-
Pseudomonas putida NCIB 9816 Q52126
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose CL-6B column chromatography and DEAE-cellulose column chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
397
-
 purified enzyme, pH and temperature not specified in the publication Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 1 month, minimal loss of activity Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH best substrate Pseudomonas putida 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricyanide + NADH best substrate Pseudomonas putida NCIB 9816 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricytochrome c + NADH
-
 Pseudomonas putida 2 ferrocytochrome c + NAD+ + H+
-
 ?
2 ferricytochrome c + NADH
-
 Pseudomonas putida NCIB 9816 2 ferrocytochrome c + NAD+ + H+
-
 ?
NADH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Pseudomonas putida NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NADH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Pseudomonas putida NCIB 9816 NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
oxidized cytochrome c + NADPH NADPH is also effective as an electron donor but yields only 39% of the activity obtained with NADH in the cytochrome c reductase assay Pseudomonas putida reduced cytochrome c + NADP+ + H+
-
 ?
oxidized cytochrome c + NADPH NADPH is also effective as an electron donor but yields only 39% of the activity obtained with NADH in the cytochrome c reductase assay Pseudomonas putida NCIB 9816 reduced cytochrome c + NADP+ + H+
-
 ?
oxidized Nitro Blue tetrazolium + NADH
-
 Pseudomonas putida reduced Nitro Blue tetrazolium + NAD+ + H+
-
 ?
oxidized Nitro Blue tetrazolium + NADH
-
 Pseudomonas putida NCIB 9816 reduced Nitro Blue tetrazolium + NAD+ + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Pseudomonas putida
monomer 1 * 37104, calculated from amino acid sequence Pseudomonas putida

Synonyms

Synonyms Comment Organism
NADH-ferredoxinNAP reductase
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 20 the purified enzyme is unstable at room temperature. It loses 50% of its cytochrome c reductase activity within the first 8 h. At 0 to 5°C, the purified enzyme retains 70% of its cytochrome c reductase activity after 5 days Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme binds 1 mol of FAD per mol of enzyme protein Pseudomonas putida
NADH
-
 Pseudomonas putida

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas putida calculated from amino acid sequence
-
 6.3

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
10
-
 pH and temperature not specified in the publication Pseudomonas putida iodoacetate