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Literature summary for 1.18.6.1 extracted from

  • Vignais, P.M.; Colbeau, A.; Willison, J.C.; Jouanneau, Y.
    Hydrogenase, nitrogenase, and hydrogen metabolism in the photosynthetic bacteria (1985), Adv. Microb. Physiol., 26, 155-234.
    View publication on PubMed
Search for more literature for 1.18.6.1

Activating Compound

Activating Compound Comment Organism Structure
additional information Fe protein contains an adenine-like molecule, a pentose moiety and a phosphate residue covalently attached to the molecule Rhodobacter capsulatus
additional information Fe protein contains an adenine-like molecule, a pentose moiety and a phosphate residue covalently attached to the molecule Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
glutamine
-
 Rhodobacter capsulatus
glutamine
-
 Rhodospirillum rubrum
NH4+ immediate inhibition, repression of induction Rhodobacter capsulatus
NH4+ immediate inhibition, repression of induction Rhodospirillum rubrum
O2
-
 Azotobacter vinelandii
O2
-
 Clostridium pasteurianum
O2
-
 Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
 Clostridium pasteurianum
Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodobacter capsulatus
Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Azotobacter vinelandii
Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodospirillum rubrum
Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Allochromatium vinosum
Iron iron content of MoFe protein: 30 Azotobacter vinelandii
Iron the MoFe protein contains 2 molybdenum, about 30 iron and 30 inorganic sulphur atoms, 16 of the 30 Fe atoms are associated with S2- in four cubic [4Fe-4S] clusters, the remaining metal atoms are arranged in two copies of a cofactor called FeMo cofactor, FeMoCo, with a minimum stoichiometry of MoFe6S8-9 Azotobacter vinelandii
Iron 2.7-4.1 mol per mol of Fe protein Rhodobacter capsulatus
Iron 20 atoms of iron per molecule of MoFe protein Rhodospirillum rubrum
Iron 27.7 atoms of iron per molecule of MoFe protein Rhodobacter capsulatus
Molybdenum
-
 Clostridium pasteurianum
Molybdenum 1.2-1.3 gatom per mol of MoFe protein Rhodobacter capsulatus
Molybdenum 1.7 gatom per mol of MoFe protein Rhodospirillum rubrum
Molybdenum 2 gatom per mol of MoFe protein Azotobacter vinelandii
Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Rhodobacter capsulatus
Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Klebsiella pneumoniae
Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Azotobacter vinelandii
Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Rhodospirillum rubrum
Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Allochromatium vinosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Clostridium pasteurianum
additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodobacter capsulatus
additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Azotobacter vinelandii
additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodospirillum rubrum
additional information
-
 enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Allochromatium vinosum
61500
-
 Fe protein Rhodospirillum rubrum
63000
-
 Fe protein Rhodobacter capsulatus
63000
-
 Fe protein Azotobacter vinelandii
230000
-
 MoFe protein Rhodobacter capsulatus
230000
-
 MoFe protein Azotobacter vinelandii
230000
-
 MoFe protein Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodobacter capsulatus
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodospirillum rubrum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Allochromatium vinosum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Allochromatium vinosum
-
-
-
Azotobacter vinelandii
-
-
-
Clostridium pasteurianum
-
-
-
Klebsiella pneumoniae
-
-
-
Rhodobacter capsulatus
-
-
-
Rhodospirillum rubrum
-
-
-

Oxidation Stability

Oxidation Stability Organism
extreme sensitivity to O2 Rhodobacter capsulatus
extreme sensitivity to O2 Klebsiella pneumoniae
extreme sensitivity to O2 Clostridium pasteurianum
extreme sensitivity to O2 Azotobacter vinelandii
extreme sensitivity to O2 Rhodospirillum rubrum
extreme sensitivity to O2 Allochromatium vinosum

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Rhodobacter capsulatus
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Klebsiella pneumoniae
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Clostridium pasteurianum
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Azotobacter vinelandii
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Rhodospirillum rubrum
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Allochromatium vinosum
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhodobacter capsulatus
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhodospirillum rubrum
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Allochromatium vinosum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Rhodobacter capsulatus 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Klebsiella pneumoniae 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Clostridium pasteurianum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Rhodospirillum rubrum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Allochromatium vinosum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Rhodobacter capsulatus 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Klebsiella pneumoniae 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Clostridium pasteurianum 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Azotobacter vinelandii 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Rhodospirillum rubrum 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
 Allochromatium vinosum 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhodobacter capsulatus 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhodospirillum rubrum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Allochromatium vinosum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Klebsiella pneumoniae
dimer
-
 Clostridium pasteurianum
dimer
-
 Azotobacter vinelandii
dimer
-
 Rhodospirillum rubrum
dimer
-
 Allochromatium vinosum
More
-
 Clostridium pasteurianum
More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodobacter capsulatus
More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Azotobacter vinelandii
More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Rhodospirillum rubrum
More enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Allochromatium vinosum
tetramer
-
 Clostridium pasteurianum
tetramer alpha: 55000, beta: 59500 Rhodobacter capsulatus
tetramer MoFe protein is an alpha2beta2-tetramer Rhodobacter capsulatus
tetramer MoFe protein is an alpha2beta2-tetramer Klebsiella pneumoniae
tetramer MoFe protein is an alpha2beta2-tetramer Azotobacter vinelandii
tetramer MoFe protein is an alpha2beta2-tetramer Rhodospirillum rubrum
tetramer MoFe protein is an alpha2beta2-tetramer Allochromatium vinosum
tetramer alpha: 58500, beta: 58500 Rhodospirillum rubrum