Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Azotobacter vinelandii | |
Mg2+ | Mg2+ required for MgATP complex | Azotobacter vinelandii | |
Molybdenum | - |
Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter vinelandii | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
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Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | MgATP/MgADP-dependent electron and proton transfer kinetics | Azotobacter vinelandii | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | MgATP-dependent | Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
dithionite + H+ + N2 + ATP | in vitro substrate | Azotobacter vinelandii | ? | - |
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Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 1200 per min: proton production of the reduced enzyme, MgATP-dependent | Azotobacter vinelandii |