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Literature summary for 1.18.6.1 extracted from

  • Erickson, J.A.; Nyborg, A.C.; Johnson, J.L.; Truscott, S.M.; Gunn, A.; Nordmeyer, F.R.; Watt, G.D.
    Enhanced efficiency of ATP hydrolysis during nitrogenase catalysis utilizing reductants That form the all-ferrous redox state of the Fe protein (1999), Biochemistry, 38, 14279-14285.
    View publication on PubMed
Search for more literature for 1.18.6.1

Inhibitors

Inhibitors Comment Organism Structure
C2H2 inhibition of H195 mutants Azotobacter vinelandii
C2H2
-
 Clostridium pasteurianum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron reduction kinetics Clostridium pasteurianum
Iron reduction kinetics Azotobacter vinelandii
Iron contains [4Fe4S] cluster Clostridium pasteurianum
Iron contains [4Fe4S] cluster Azotobacter vinelandii
Mg2+ Mg2+ required for MgATP complex Clostridium pasteurianum
Mg2+ Mg2+ required for MgATP complex Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii OP
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Azotobacter vinelandii OP
-
-
-
Clostridium pasteurianum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum
a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter vinelandii OP 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
dithionite + H+ + N2 + ATP in vitro substrate Clostridium pasteurianum ?
-
 ?
dithionite + H+ + N2 + ATP in vitro substrate Azotobacter vinelandii ?
-
 ?
dithionite + H+ + N2 + ATP in vitro substrate Azotobacter vinelandii OP ?
-
 ?
Ti4+ + H+ + N2 + ATP in vitro substrate Clostridium pasteurianum ?
-
 ?
Ti4+ + H+ + N2 + ATP in vitro substrate Azotobacter vinelandii ?
-
 ?
Ti4+ + H+ + N2 + ATP in vitro substrate Azotobacter vinelandii OP ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15 40 no maximum with Ti3+ as reductant Azotobacter vinelandii
30
-
 assay at Clostridium pasteurianum
30
-
 assay at Azotobacter vinelandii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40 no maximum with Ti3+ as reductant Azotobacter vinelandii