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Literature summary for 1.18.6.1 extracted from

  • Cui, Z.; Dunford, A.J.; Durrant, M.C.; Henderson, R.A.; Smith, B.E.
    Binding sites of nitrogenase: kinetic and theoretical studies of cyanide binding to extracted FeMo-cofactor derivatives (2003), Inorg. Chem., 42, 6252-6264.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cyanide inhibits electron flow Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information reaction kinetics for the isolated MoFe protein in reaction with cyanide with ligands tert-butylcyanide, butyl isocyanide, or imidazole, bound to the cofactor protein, cyanide binding kinetics, stopped-flow measurements Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Iron dependent on, the enzyme complex contains a molybdenum-iron protein, and a dimeric iron protein Klebsiella pneumoniae
Molybdenum dependent on, the enzyme complex contains a molybdenum-iron protein harboring the active site Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced ferredoxin + H+ + N2 + ATP + H2O Klebsiella pneumoniae
-
oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
ir
reduced ferredoxin + H+ + N2 + ATP + H2O Klebsiella pneumoniae M5a1
-
oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae M5a1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of the enzyme complex and isolation of the MoFe protein thereof Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate the active site is located on the MoFe protein, active site structure and substrate binding mechanism Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylene + ?
-
Klebsiella pneumoniae ethylene + ?
-
?
acetylene + ?
-
Klebsiella pneumoniae M5a1 ethylene + ?
-
?
cyanide + ? cyanide favors the molydenum for binding Klebsiella pneumoniae ?
-
?
cyanide + ? cyanide favors the molydenum for binding Klebsiella pneumoniae M5a1 ?
-
?
cyclopropene + ?
-
Klebsiella pneumoniae ?
-
?
cyclopropene + ?
-
Klebsiella pneumoniae M5a1 ?
-
?
ethylene + ?
-
Klebsiella pneumoniae ?
-
?
ethylene + ?
-
Klebsiella pneumoniae M5a1 ?
-
?
methyl isocyanide + ?
-
Klebsiella pneumoniae ?
-
?
N2O + ?
-
Klebsiella pneumoniae ?
-
?
N3- + ?
-
Klebsiella pneumoniae ?
-
?
reduced ferredoxin + H+ + N2 + ATP + H2O
-
Klebsiella pneumoniae oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
ir
reduced ferredoxin + H+ + N2 + ATP + H2O
-
Klebsiella pneumoniae M5a1 oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Klebsiella pneumoniae
Ferredoxin
-
Klebsiella pneumoniae
iron-molybdenum cofactor
-
Klebsiella pneumoniae