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Literature summary for 1.18.6.1 extracted from

  • Masukawa, H.; Inoue, K.; Sakurai, H.; Wolk, C.P.; Hausinger, R.P.
    Site-directed mutagenesis of the Anabaena sp. strain PCC 7120 nitrogenase active site to increase photobiological hydrogen production (2010), Appl. Environ. Microbiol., 76, 6741-6750.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant gene nifD by gene replacement, Escherichia coli strain HB101(pRL623) and J53 (RP4) are used for the transfer of mobilizable plasmids into Anabaena cells, with a deletion of nifHDK-ORF, by conjugation via triparental mating, subcloning in Escherichia coli strain XL-1 Blue Anabaena sp.

Protein Variants

Protein Variants Comment Organism
F388 A site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
F388H site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
F388T site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
F388Y site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197A site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197D site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197E site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197F site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197G site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197K site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197L site-directed mutagenesis, a variant of NifDDELTAH, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overviewup Anabaena sp.
H197N site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197Q site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197R site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197S site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197T site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
H197Y site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
additional information engineering of cyanobacterial strains for enhanced photobiological production of H2 in an aerobic, nitrogen-containing environment, overview Anabaena sp.
Q193A site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Q193G site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Q193H site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Q193K site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Q193L site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Q193N site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Q193S site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Q193V site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284C site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284E site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284F site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284H site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284K site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284L site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284Q site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284T site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
R284Y site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
S285A site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
S285C site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
S285D site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
S285G site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
S285M site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
S285N site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
S285Q site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
S285T site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Y236A site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Y236D site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Y236F site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Y236H site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Y236M site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Y236N site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.
Y236T site-directed mutagenesis, a variant of NifDDELTAHup, nitrogen-fixing ability, H2 production rate, and reduction rate of acetylene (under Ar) of the mutant compared to the NifD control, overview Anabaena sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Anabaena sp.
Molybdenum
-
Anabaena sp.

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
-
-
Anabaena sp. PCC 7120
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme is localized to the microaerobic environment of heterocysts, a highly differentiated subset of the filamentous cells Anabaena sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
comparison of relative nitrogen-fixing ability, H2 production rates, and reduction rates of acetylene (under Ar) of wild-type and mutant NifD variants, overview Anabaena sp.

Synonyms

Synonyms Comment Organism
molybdenum-containing nitrogenase
-
Anabaena sp.

Cofactor

Cofactor Comment Organism Structure
ATP the Fe protein is a homodimer containing a single [4Fe-4S] cluster and functions as an ATP-dependent electron donor to the MoFe protein Anabaena sp.
FeMo cofactor bound at the active site, the MoFe protein, encoded by nifD and nifK. It is an alpha2beta2 heterotetramer with each nifD-encoded alpha subunit coordinating the FeMo cofactor that binds and reduces substrate, while alpha plus the nifK-encoded beta subunits coordinate the [8Fe-7S] P-cluster Anabaena sp.
iron cofactor the Fe protein is a homodimer containing a single [4Fe-4S] cluster and functions as an ATP-dependent electron donor to the MoFe protein Anabaena sp.

General Information

General Information Comment Organism
additional information nitrogenase consists of the Fe protein, encoded by nifH, and the MoFe protein, encoded by nifD and nifK. The Fe protein is a homodimer containing a single [4Fe-4S] cluster and functions as an ATP-dependent electron donor to the MoFe protein, which is bound at the active site and alpha2beta2 heterotetramer with each nifD-encoded alpha subunit coordinating the FeMo cofactor that binds and reduces substrate, while alpha plus the nifK-encoded beta subunits coordinate the [8Fe-7S] P-cluster Anabaena sp.
physiological function nitrogenase, an oxygen-labile enzyme typically containing an iron-molybdenum cofactor active site, is responsible for nitrogen fixation producing photobiological H2 as a byproduct Anabaena sp.