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Literature summary for 1.2.1.10 extracted from

  • Powlowski, J.; Sahlman, L.; Shingler, V.
    Purification and properties of the physically associated meta-cleavage pathway enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600 (1993), J. Bacteriol., 175, 377-385.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pseudomonas sp.

General Stability

General Stability Organism
activity is highest in HEPES buffer and somewhat lower in phosphate buffer, activity in Tris buffer is about half the rate in HEPES buffer Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
2 * 32500 + 2 * 39000, determined by nucleotide sequence, SDS-PAGE, two subunits of aldehyde dehydrogenase (acylating) and two of 4-hydroxy-2-ketovalerate Pseudomonas sp.
39000
-
2 * 32500 + 2 * 39000, determined by nucleotide sequence, SDS-PAGE, two subunits of aldehyde dehydrogenase (acylating) and two of 4-hydroxy-2-ketovalerate Pseudomonas sp.
148000
-
gel filtration, molecular mass of enzyme complex, occurs in complex with 4-hydroxy-2-ketovalerate Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + CoA + NAD+ Pseudomonas sp.
-
acetyl-CoA + NADH
-
?
acetaldehyde + CoA + NAD+ Pseudomonas sp. CF 600
-
acetyl-CoA + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. CF 600
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
-80°C, 10 mM phosphate buffer, pH 7.5, 1 mM dithiothreitol Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
Pseudomonas sp. acetyl-CoA + NADH
-
?
acetaldehyde + CoA + NAD+
-
Pseudomonas sp. CF 600 acetyl-CoA + NADH
-
?
formaldehyde + CoA + NAD+
-
Pseudomonas sp. formyl-CoA + NADH
-
?
formaldehyde + CoA + NAD+
-
Pseudomonas sp. CF 600 formyl-CoA + NADH
-
?
isobutyraldehyde + CoA + NAD+
-
Pseudomonas sp. isobutyryl-CoA + NADH
-
?
isobutyraldehyde + CoA + NAD+
-
Pseudomonas sp. CF 600 isobutyryl-CoA + NADH
-
?
n-butyraldehyde + CoA + NAD+
-
Pseudomonas sp. n-butyryl-CoA + NADH
-
?
n-butyraldehyde + CoA + NAD+
-
Pseudomonas sp. CF 600 n-butyryl-CoA + NADH
-
?
propanal + CoA + NAD+ initial rate of reaction with propanal is 2.7fold slower than that with acetaldehyde Pseudomonas sp. propionyl-CoA + NADH
-
?
propanal + CoA + NAD+ initial rate of reaction with propanal is 2.7fold slower than that with acetaldehyde Pseudomonas sp. CF 600 propionyl-CoA + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 32500 + 2 * 39000, determined by nucleotide sequence, SDS-PAGE, two subunits of aldehyde dehydrogenase (acylating) and two of 4-hydroxy-2-ketovalerate Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 activity increases gradually over pH range from 6.5 to 8.5 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
CoA
-
Pseudomonas sp.
NAD+
-
Pseudomonas sp.
NADH rate of reaction with NADP+ is only 7% of that with NAD+ Pseudomonas sp.