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Literature summary for 1.2.1.10 extracted from

  • Krutsakorn, B.; Imagawa, T.; Honda, K.; Okano, K.; Ohtake, H.
    Construction of an in vitro bypassed pyruvate decarboxylation pathway using thermostable enzyme modules and its application to N-acetylglutamate production (2013), Microb. Cell Fact., 12, 91.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis construction of a bypassed pyruvate decarboxylation pathway, through which pyruvate can be converted to acetyl-CoA, by using a coupled enzyme system consisting of pyruvate decarboxylase from Acetobacter pasteurianus and the CoA-acylating aldehyde dehydrogenase from Thermus thermophilus. A cofactor-balanced and CoA-recycling synthetic pathway for N-acetylglutamate production is designed by coupling the bypassed pathway with the glutamate dehydrogenase from Thermus thermophilus and N-acetylglutamate synthase from Thermotoga maritima. N-Acetylglutamate can be produced from an equimolar mixture of pyruvate and alpha-ketoglutarate with a molar yield of 55% through the synthetic pathway consisting of a mixture of four recombinant Escherichia coli strains having either one of the thermostable enzymes. The overall recycling number of CoA is 27 Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q53WH9
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Thermus thermophilus DSM 579 Q53WH9
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Synonyms

Synonyms Comment Organism
TTHB247
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Thermus thermophilus