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Literature summary for 1.2.1.11 extracted from
- Cloning and characterization of aspartate-beta-semialdehyde dehydrogenase from Mycobacterium tuberculosis H37 Rv (2005), J. Appl. Microbiol., 98, 832-838.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is a validated drug target | Mycobacterium tuberculosis |
| pharmacology | inhibitor design from enzyme three-dimensional structure | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene asd, subcloning in Escherichia coli strain JM109, DNA sequence determination and analysis, optimization of functional overexpression in Escherichia coli strain M15 as His-tagged protein | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
NADP+ | recombinant enzyme, pH 9.0, 30°C | Mycobacterium tuberculosis |  |
| 0.955 | - |
L-aspartate-4-semialdehyde | recombinant enzyme, pH 9.0, 30°C | Mycobacterium tuberculosis | |
| 11.39 | - |
phosphate | recombinant enzyme, pH 9.0, 30°C | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, about, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-4-aspartyl phosphate + NADPH | Mycobacterium tuberculosis | physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
| L-4-aspartyl phosphate + NADPH | Mycobacterium tuberculosis H37Rv | physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WNX5 | strain H37 Rv, gene asd | - |
| Mycobacterium tuberculosis H37Rv | P9WNX5 | strain H37 Rv, gene asd | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to 96% purity | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
purified recombinant enzyme | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-4-aspartyl phosphate + NADPH | physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway | Mycobacterium tuberculosis | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
| L-4-aspartyl phosphate + NADPH | physiological forward reaction direction, key enzyme in diaminopimelic acid biosynthetic pathway | Mycobacterium tuberculosis H37Rv | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
? | |
| L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
Mycobacterium tuberculosis | L-4-aspartyl phosphate + NADPH | - |
r | |
| L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
Mycobacterium tuberculosis H37Rv | L-4-aspartyl phosphate + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, about, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ASD enzyme | - |
Mycobacterium tuberculosis |
| aspartate-beta-semialdehyde dehydrogenase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.49 | - |
NADP+ | recombinant enzyme, pH 9.0, 30°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Mycobacterium tuberculosis | |
| NADPH | - |
Mycobacterium tuberculosis | |
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