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Literature summary for 1.2.1.11 extracted from

  • Vyas, R.; Kumar, V.; Panjikar, S.; Karthikeyan, S.; Kishan, K.V.; Tewari, R.; Weiss, M.S.
    Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis (2008), Acta Crystallogr. Sect. F, 64, 167-170.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli strain M15 Mycobacterium tuberculosis
heterologous expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
by using the hanging-drop vapour-diffusion method, crystallization in 2 different crystal forms, diffraction data analysis suggests the presence of up to four monomers in the asymmetric unit of the orthorhombic crystal form and of one or 2 monomers in the cubic crystal form Mycobacterium tuberculosis
purified recombinant His-tagged enzyme, 9 mg/ml protein in 10 mM potassium phosphate buffer, pH 8.0, and 10 mM DTT, sitting drop vapour diffusion method, mixing of 500 nl of protein and of reservoir solution, the latter containing 1.6 M ammonium sulfate and 100 mM citric acid, pH 5.0, 2 days at 18°C, two different crystal forms, X-ray diffraction structure determination and analysis at 2.18-2.75 A resolution Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, about, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis
38070
-
the expressed and purified monomer protein construct includes the 17-amino-acid sequence RGSHHHHHHGSACELGT between the N-terminal Met and the second amino acid Gly of the native Asd sequence, the total length of this protein is 362 amino acids Mycobacterium tuberculosis
75000
-
recombinant His-tagged enzyme, gel filtration Mycobacterium tuberculosis
75000
-
homodimer in solution, determined by gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aspartate-4-semialdehyde + phosphate + NADP+ Mycobacterium tuberculosis second enzyme in the lysine/homoserine biosynthetic pathways L-4-aspartyl phosphate + NADPH + H+
-
r
aspartate-4-semialdehyde + phosphate + NADP+ Mycobacterium tuberculosis H37Rv second enzyme in the lysine/homoserine biosynthetic pathways L-4-aspartyl phosphate + NADPH + H+
-
r
L-aspartate 4-semialdehyde + phosphate + NADP+ Mycobacterium tuberculosis the enzyme takes part in the lysine/homoserine-biosynthetic pathway L-4-aspartyl phosphate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
gene Rv3708c
-
Mycobacterium tuberculosis P9WNX5
-
-
Mycobacterium tuberculosis H37Rv P9WNX5
-
-

Purification (Commentary)

Purification (Comment) Organism
by using affinity chromatography and gel filtration Mycobacterium tuberculosis
recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aspartate-4-semialdehyde + phosphate + NADP+ second enzyme in the lysine/homoserine biosynthetic pathways Mycobacterium tuberculosis L-4-aspartyl phosphate + NADPH + H+
-
r
aspartate-4-semialdehyde + phosphate + NADP+ second enzyme in the lysine/homoserine biosynthetic pathways Mycobacterium tuberculosis H37Rv L-4-aspartyl phosphate + NADPH + H+
-
r
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Mycobacterium tuberculosis L-4-aspartyl phosphate + NADPH + H+
-
?
L-aspartate 4-semialdehyde + phosphate + NADP+ the enzyme takes part in the lysine/homoserine-biosynthetic pathway Mycobacterium tuberculosis L-4-aspartyl phosphate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37000, about, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis
homodimer recombinant enzyme Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
ASADH
-
Mycobacterium tuberculosis
Asd
-
Mycobacterium tuberculosis
aspartate semialdehyde dehydrogenase
-
Mycobacterium tuberculosis
aspartate-beta-semialdehyde dehydrogenase
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Mycobacterium tuberculosis