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Literature summary for 1.2.1.12 extracted from

  • Lambeir, A.M.; Loiseau, A.M.; Kuntz, D.A.; Vellieux, F.M.; Michels, P.A.M.; Opperdoes, F.R.
    The cytosolic and glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei. Kinetic properties and comparison with homologous enzymes (1991), Eur. J. Biochem., 198, 429-435.
    View publication on PubMed
Search for more literature for 1.2.1.12

Inhibitors

Inhibitors Comment Organism Structure
Agaricic acid 0.14 mM, 50% inhibition in absence of NAD+. 2 mM, 35% loss of activity in presence of 0.017 mM NAD+ Oryctolagus cuniculus
Agaricic acid glyoxysomal enzyme: 0.2 mM, 60% loss of activity. Cytosolic enzyme: 2 mM, less than 20% loss of activity Trypanosoma brucei
gossypol
-
 Trypanosoma brucei
pentalenolactone reversible Geobacillus stearothermophilus
pentalenolactone irreversible Oryctolagus cuniculus
pentalenolactone most potent inhibitor; reversible Trypanosoma brucei
suramin cyosolic enzyme: competitive with NAD+. Effect on Km-value and maximal veocity of glyoxysomal enzyme Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 NADH cytosolic enzyme Trypanosoma brucei
0.01
-
 NADH
-
 Homo sapiens
0.012
-
 NADH
-
 Oryctolagus cuniculus
0.012
-
 NADH
-
 Geobacillus stearothermophilus
0.02
-
 NADH glyoxysomal enzyme Trypanosoma brucei
0.034
-
 3-phospho-D-glyceroyl phosphate
-
 Geobacillus stearothermophilus
0.039
-
 NAD+
-
 Geobacillus stearothermophilus
0.04
-
 NAD+ cytosolic enzyme Trypanosoma brucei
0.05
-
 NAD+
-
 Homo sapiens
0.05
-
 NAD+
-
 Geobacillus stearothermophilus
0.06
-
 NAD+
-
 Oryctolagus cuniculus
0.07
-
 D-glyceraldehyde 3-phosphate
-
 Homo sapiens
0.082
-
 D-glyceraldehyde 3-phosphate
-
 Oryctolagus cuniculus
0.09
-
 D-glyceraldehyde 3-phosphate
-
 Oryctolagus cuniculus
0.09
-
 D-glyceraldehyde 3-phosphate
-
 Geobacillus stearothermophilus
0.1
-
 3-phospho-D-glyceroyl phosphate glyoxysomal enzyme Trypanosoma brucei
0.13
-
 3-phospho-D-glyceroyl phosphate
-
 Oryctolagus cuniculus
0.14
-
 3-phospho-D-glyceroyl phosphate
-
 Homo sapiens
0.14
-
 3-phospho-D-glyceroyl phosphate cytosolic enzyme Trypanosoma brucei
0.15
-
 D-glyceraldehyde 3-phosphate glyoxysomal enzyme Trypanosoma brucei
0.17
-
 D-glyceraldehyde 3-phosphate cytosolic enzyme Trypanosoma brucei
0.45
-
 NAD+ glyoxysomal enzyme Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Trypanosoma brucei 5829
-
glyoxysome
-
 Trypanosoma brucei 9514
-

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cytosolic isoenzyme Trypanosoma brucei

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
muscle
-
 Homo sapiens
-
muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Geobacillus stearothermophilus
additional information
-
-
 Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH
-
 Homo sapiens D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
3-phospho-D-glyceroyl phosphate + NADH
-
 Oryctolagus cuniculus D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
3-phospho-D-glyceroyl phosphate + NADH
-
 Geobacillus stearothermophilus D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
3-phospho-D-glyceroyl phosphate + NADH
-
 Trypanosoma brucei D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Homo sapiens 3-phospho-D-glyceroyl phosphate + NADH
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + NADH
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Geobacillus stearothermophilus 3-phospho-D-glyceroyl phosphate + NADH
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Trypanosoma brucei 3-phospho-D-glyceroyl phosphate + NADH
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 cytosolic enzyme and enzyme from glyoxysomes Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NAD+
-
 Oryctolagus cuniculus
NAD+
-
 Geobacillus stearothermophilus
NAD+
-
 Trypanosoma brucei
NADH cofactor Homo sapiens
NADH cofactor Oryctolagus cuniculus
NADH cofactor Geobacillus stearothermophilus
NADH cofactor Trypanosoma brucei