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Literature summary for 1.2.1.12 extracted from

  • Egea, L.; Aguilera, L.; Gimenez, R.; Sorolla, M.A.; Aguilar, J.; Badia, J.; Baldoma, L.
    Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: interaction of the extracellular enzyme with human plasminogen and fibrinogen (2007), Int. J. Biochem. Cell Biol., 39, 1190-1203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted GAPDH is located on the bacterial surface and released to the culture medium of enterohemorrhagic and enteropathogenic strains, secreted GAPDH remains associated with colonic Caco-2 epithelial cells after adhesion and binds human plasminogen and fribinogen, non-pathogenic Escherichia coli strains do not secrete GAPDH Escherichia coli
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
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SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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enterohemorrhagic strain 86-24h11and enteropathogenic strain E2348/69
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Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose 4B resin column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
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Escherichia coli 3-phospho-D-glyceroyl phosphate + NADH + H+
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?

Synonyms

Synonyms Comment Organism
GAPDH
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Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
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Escherichia coli