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Literature summary for 1.2.1.12 extracted from
- Molecular characterization of tumor associated glyceraldehyde-3-phosphate dehydrogenase (2009), Biochemistry (Moscow), 74, 717-727.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | glyceraldehyde 3-phosphate dehydrogenase from malignant sources shows altered properties compared with enzyme from healthy subjects | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | inhibition is less pronounced for enzyme from sarcoma tissue as compared to normal muscle tissue | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
NAD+ | pH 7.3, enzyme isolated of sarcoma tissue | Homo sapiens | |
| 0.036 | - |
NAD+ | pH 8.0, enzyme isolated of sarcoma tissue | Homo sapiens | |
| 0.04 | - |
NAD+ | pH 8.8, enzyme isolated of sarcoma tissue | Homo sapiens | |
| 0.047 | - |
NAD+ | pH 8.8, enzyme isolated of healthy patients | Homo sapiens | |
| 0.062 | - |
NAD+ | pH 8.0, enzyme isolated of healthy patients | Homo sapiens | |
| 0.071 | - |
NAD+ | pH 7.3, enzyme isolated of healthy patients | Homo sapiens | |
| 0.087 | - |
D-glyceraldehyde 3-phosphate | pH 7.3, enzyme isolated of sarcoma tissue | Homo sapiens | |
| 0.089 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, enzyme isolated of sarcoma tissue | Homo sapiens | |
| 0.11 | - |
D-glyceraldehyde 3-phosphate | pH 8.8, enzyme isolated of sarcoma tissue | Homo sapiens | |
| 0.149 | - |
D-glyceraldehyde 3-phosphate | pH 7.3, enzyme isolated of healthy patients | Homo sapiens | |
| 0.159 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, enzyme isolated of healthy patients | Homo sapiens | |
| 0.16 | - |
D-glyceraldehyde 3-phosphate | pH 8.8, enzyme isolated of healthy patients | Homo sapiens | |
| 4 | - |
phosphate | pH 8.8, enzyme isolated of healthy patients | Homo sapiens | |
| 9.9 | - |
phosphate | pH 8.8, enzyme isolated of sarcoma tissue | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from normal leukocytes of healthy subjects, leukocytes of chronic myeloid leukemia patients and from sarcoma tissue | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| at -20°C storage after dialysis following the first (NH4)2SO4 fractionation step during purification, normal muscle and leukocyte enzyme is stable. Enzyme from chronic myeloid leukemia patients and sarcoma tissue loses about 80% of activity. | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8.8 | enzyme isolated from sarcoma tissue, broad | Homo sapiens |
| 8.6 | - |
enzyme isolated from normal tissue, sharp optimum | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
above, sharp decline in activity for enzyme of patients with chronic myeloid leukemia, no similar sharp decline for enzyme of healthy subjects | Homo sapiens |
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Release 2023.1 (January 2023)
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