Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.12 extracted from

  • Vescovi, M.; Zaffagnini, M.; Festa, M.; Trost, P.; Lo Schiavo, F.; Costa, A.
    Nuclear accumulation of cytosolic glyceraldehyde-3-phosphate dehydrogenase in cadmium-stressed Arabidopsis roots (2013), Plant Physiol., 162, 333-346.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.12

Application

Application Comment Organism
agriculture cadmium-induced stress in seedlings roots induces nitric oxide accumulation, cytosolic oxidation, activation of the GAPC1 promoter, GAPC1 protein accumulation in enzymatically inactive form, and strong relocalization of GAPC1 to the nucleus. All the effects are detected in the same zone of the root tip. In vitro, GAPC1 is inactivated by either nitric oxide donors or hydrogen peroxide, but no inhibition is directly provided by cadmium Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C155S nuclear relocalization of GAPC1 under cadmium-induced oxidative stress is stimulated, rather than inhibited, by mutation of the catalytic cysteine C155 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
H2O2 isoform GAPC1 is irreversibly inhibited in a time- and concentration-dependent manner Arabidopsis thaliana
nitric oxide isoform GAPC1 is irreversibly inhibited in a time- and concentration-dependent manner Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-
nucleus relocalization of GAPC1 to the nucleus is induced by calcium stress Arabidopsis thaliana 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Arabidopsis thaliana
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Columbia
-
Arabidopsis thaliana P25858
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Arabidopsis thaliana 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GapC1
-
 Arabidopsis thaliana
GapC1 isoform Arabidopsis thaliana
GapC2 isoform Arabidopsis thaliana
GAPDH
-
 Arabidopsis thaliana
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana 72 h cadmium treatment has no stimulating effect on the extractable NADH-specific glyceraldehyde-3-phosphate dehydrogenase activity of whole seedlings additional information
Arabidopsis thaliana cadmium induces isoform GAPC1 accumulation in an inactive form. L-buthionine-sulfoximine induces both isoform GAPC1 promoter activity and protein accumulation in the seedling root tips up

General Information

General Information Comment Organism
physiological function cadmium-induced stress in seedlings roots induces nitric oxide accumulation, cytosolic oxidation, activation of the GAPC1 promoter, GAPC1 protein accumulation in enzymatically inactive form, and strong relocalization of GAPC1 to the nucleus. All the effects are detected in the same zone of the root tip. In vitro, GAPC1 is inactivated by either nitric oxide donors or hydrogen peroxide, but no inhibition is directly provided by cadmium Arabidopsis thaliana