General Stability | Organism |
---|---|
it cannot be stabilized with sorbitol, glucose, polyethylene glycol 400, beta-alanine, glycine or glycerol | Haloarcula vallismortis |
stable for many days in all buffers, containing more than 2 mol/l salt | Haloarcula vallismortis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | 1 | NAD+ | 45°C, pH 7.8 | Haloarcula vallismortis | |
1.25 | - |
phosphate | 45°C, pH 7.8 | Haloarcula vallismortis | |
8.3 | - |
arsenate | 45°C, pH 7.8 | Haloarcula vallismortis | |
15 | - |
D-glyceraldehyde 3-phosphate | 45°C, pH 7.8 | Haloarcula vallismortis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | exhibits its highest activity in 2 mol/l KCl at 45°C | Haloarcula vallismortis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, cetyltrimethyl ammonium bromide PAGE | Haloarcula vallismortis |
160000 | - |
gel filtration | Haloarcula vallismortis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula vallismortis | - |
- |
- |
Haloarcula vallismortis ATCC 29715 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula vallismortis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + arsenate + NAD+ | strictly NAD+-dependent enzyme. The maximal velocity for the reaction with arsenate instead of phosphate is about 10 times higher. The maximal reaction velocity is higher with arsenate because the degradation of the instable product phospho-arseno glyceric acid pushes the reaction into the direction of phospho-arseno glyceric acid formation | Haloarcula vallismortis | 3-phospho-D-glyceroyl arsenate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + arsenate + NAD+ | strictly NAD+-dependent enzyme. The maximal velocity for the reaction with arsenate instead of phosphate is about 10 times higher. The maximal reaction velocity is higher with arsenate because the degradation of the instable product phospho-arseno glyceric acid pushes the reaction into the direction of phospho-arseno glyceric acid formation | Haloarcula vallismortis ATCC 29715 | 3-phospho-D-glyceroyl arsenate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | strictly NAD+-dependent enzyme | Haloarcula vallismortis | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | strictly NAD+-dependent enzyme | Haloarcula vallismortis ATCC 29715 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, cetyltrimethyl ammonium bromide PAGE | Haloarcula vallismortis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the enzyme exhibits its highest activity in 2 mol/l KCl at 45°C | Haloarcula vallismortis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
activity is lost after several min below 15°C | Haloarcula vallismortis |
22 | - |
at room temperature the enzyme is stable for several weeks | Haloarcula vallismortis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Haloarcula vallismortis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly NAD+-dependent enzyme. No acitivity with NADP + as coenzyme can be detected | Haloarcula vallismortis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Haloarcula vallismortis | isoelectric focusing | - |
4.25 |