Protein Variants | Comment | Organism |
---|---|---|
additional information | a downregulation of GAPDH activity does not contribute to improved performance of engineered Saccharomyces cerevisiae on pentose substrates | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
D-glyceraldehyde 3-phosphate | isoform TDH3, pH 8.5, 25°C | Saccharomyces cerevisiae | |
0.42 | - |
D-glyceraldehyde 3-phosphate | isoform TDH2, pH 8.5, 25°C | Saccharomyces cerevisiae | |
0.86 | - |
D-glyceraldehyde 3-phosphate | isoform TDH1, pH 8.5, 25°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Saccharomyces cerevisiae | 3-phospho-D-glyceroyl phosphate + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TDH1 | - |
Saccharomyces cerevisiae |
TDH2 | - |
Saccharomyces cerevisiae |
TDH3 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
D-glyceraldehyde 3-phosphate | isoform TDH3, pH 8.5, 25°C | Saccharomyces cerevisiae | |
16 | - |
D-glyceraldehyde 3-phosphate | isoform TDH2, pH 8.5, 25°C | Saccharomyces cerevisiae | |
29 | - |
D-glyceraldehyde 3-phosphate | isoform TDH1, pH 8.5, 25°C | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | deletion mutants of isoforms TDH1, TDH2, TDH3 show decreased enzymic activity following the trend ?tdh3>?tdh2>?tdh1 in both YEPD or YEPE medium. TDH3 encodes the major GAPDH isoenzyme. The GAPDH total activity is significantly lower in all genotypes grown on ethanol in comparison with the activity on glucose. A downregulation of GAPDH activity does not contribute to improved performance of engineered Saccharomyces cerevisiae on pentose substrates | Saccharomyces cerevisiae |