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Literature summary for 1.2.1.13 extracted from
- Molecular forms of chloroplast glyceraldehyde-3-P-dehydrogenase (1983), Experientia, 39, 50-52.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Spinacia oleracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
GAPDH III, gel filtration | Spinacia oleracea |
| 37000 | - |
x * 43000 + x * 37000, molar ratio of the subunits is 1:1, GAPDH I, SDS-PAGE | Spinacia oleracea |
| 37000 | - |
x * 43000 + x * 37000, excess of the lighter subunit, GAPDH II, SDS-PAGE | Spinacia oleracea |
| 37000 | - |
4 * 37000, GAPDH III, SDS-PAGE | Spinacia oleracea |
| 43000 | - |
x * 43000 + x * 37000, molar ratio of the subunits is 1:1, GAPDH I, SDS-PAGE | Spinacia oleracea |
| 43000 | - |
x * 43000 + x * 37000, excess of the lighter subunit, GAPDH II, SDS-PAGE | Spinacia oleracea |
| 300000 | - |
GAPDH II, gel filtration | Spinacia oleracea |
| 600000 | - |
GAPDH I, gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 enzyme forms: GAPDH I, GAPDH II, GAPDH III | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, GAPDH I is stable for several months, GAPDH II and GAPDH III spontaneously lose activity | Spinacia oleracea |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000 + x * 37000, molar ratio of the subunits is 1:1, GAPDH I, SDS-PAGE | Spinacia oleracea |
| ? | x * 43000 + x * 37000, excess of the lighter subunit, GAPDH II, SDS-PAGE | Spinacia oleracea |
| More | - |
Spinacia oleracea |
| tetramer | 4 * 37000, GAPDH III, SDS-PAGE | Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | activity with NADP+ is higher than with NAD+ | Spinacia oleracea |  |
| NADP+ | cofactor | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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