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Literature summary for 1.2.1.13 extracted from

  • Kouril, T.; Esser, D.; Kort J.; Westerhoff, H.V.; Siebers, B.; Snoep, J.L.
    Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus (2013), FEBS J., 280, 4666-4680.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00041
-
3-phospho-D-glyceroyl phosphate pH 6.5, 70°C Saccharolobus solfataricus
0.074
-
NADPH pH 6.5, 70°C Saccharolobus solfataricus
0.271
-
NADP+ pH 6.5, 70°C Saccharolobus solfataricus
0.838
-
D-glyceraldehyde 3-phosphate pH 6.5, 70°C Saccharolobus solfataricus
409
-
phosphate pH 6.5, 70°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P39460
-
-
Saccharolobus solfataricus P2 P39460
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
Saccharolobus solfataricus 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
Saccharolobus solfataricus P2 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
GAPDH
-
Saccharolobus solfataricus
SSO0528 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function gluconeogenic pathway Saccharolobus solfataricus