Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
3 * 75000, SDS-PAGE | Pseudomonas sp. |
228000 | - |
gel filtration, PAGE | Pseudomonas sp. |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | stable in 30% with 5 mM mercaptoethanol + 5 mM EDTA | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from putrescine-grown cells, using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, Sephadex G-200, Hydroxylapatite and Sephadex G-200 in 150 mM NaCl | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanal + NAD+ + H2O | - |
Pseudomonas sp. | 4-aminobutanoate + NADH + H+ | - |
? | |
additional information | very low activity with: succinate semialdehyde | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 75000, SDS-PAGE | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
3-aminopropanal | Pseudomonas sp. |
9 | 10 | 4-aminobutanal | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |