Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% inhibition at 5 mM | Escherichia coli | |
Cu2+ | 50% inhibition at 0.25 mM | Escherichia coli | |
glyceraldehyde | substrate inhibition | Escherichia coli | |
glycolaldehyde | substrate inhibition | Escherichia coli | |
lactaldehyde | substrate inhibition | Escherichia coli | |
methylglyoxal | substrate inhibition | Escherichia coli | |
Mn2+ | 50% inhibition at 10 mM | Escherichia coli | |
p-hydroxymercuribenzoate | 50% inhibition at 1.5 mM | Escherichia coli | |
Zn2+ | 50% inhibition at 0.4 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-lactaldehyde | - |
Escherichia coli | |
0.12 | - |
NAD+ | + L-lactaldehyde | Escherichia coli | |
0.15 | - |
L-glyceraldehyde | - |
Escherichia coli | |
0.28 | - |
NAD+ | cosubstrate glycolaldehyde | Escherichia coli | |
0.38 | - |
glycolaldehyde | - |
Escherichia coli | |
1 | - |
methylglyoxal | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Escherichia coli |
220000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactaldehyde + NAD+ | Escherichia coli | general role in aldehyde oxidation, involved in several metabolic pathways | (S)-lactate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
inducible under aerobic conditions | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactaldehyde + NAD+ | general role in aldehyde oxidation, involved in several metabolic pathways | Escherichia coli | (S)-lactate + NADH | - |
? | |
(S)-lactaldehyde + NAD+ + H2O | - |
Escherichia coli | (S)-lactate + NADH + H+ | - |
ir | |
glycolaldehyde + NAD+ + H2O | - |
Escherichia coli | glycolate + NADH | - |
ir | |
L-glyceraldehyde + NAD+ + H2O | - |
Escherichia coli | L-glycerate + NADH | - |
ir | |
methylglyoxal + NAD+ + H2O | - |
Escherichia coli | pyruvate + NADH | - |
ir | |
additional information | oxidizes several aldehydes, e.g. L-glyceraldehyde, glycolaldehyde, methylglyoxal | Escherichia coli | ? | - |
? | |
additional information | not: acetaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | four NAD-binding sites per molecule of native enzyme | Escherichia coli |