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Literature summary for 1.2.1.23 extracted from
- alpha-Keto aldehyde dehydrogenase, an enzyme that catalyzes the enzymic oxidation of methylglyoxal to pyruvate (1967), J. Biol. Chem., 242, 4603-4609.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| barbiturates | - |
Ovis aries | |
| cyanide | - |
Ovis aries |  |
| glycine buffer | complete blockage of the reaction | Ovis aries | |
| hydrazine | 1-3 mM | Ovis aries | |
| hydroxylamine | 1-3 mM | Ovis aries | |
| N-ethylmaleimide | 3 mM | Ovis aries | |
| N-tris(hydroxymethyl)methylglycine | - |
Ovis aries | |
| p-chloromercuribenzoate | - |
Ovis aries | |
| p-hydroxymercuribenzoate | 1-3 mM | Ovis aries | |
| Semicarbazide | 1-3 mM | Ovis aries | |
| Sodium bisulfite | 1 mM | Ovis aries | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methylglyoxal + NAD+ + H2O | Ovis aries | ir | pyruvate + NADH + H+ | - |
? | |
| methylglyoxal + NAD+ + H2O | Ovis aries | i.e. pyruvaldehyde | pyruvate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using acetone powder extract, ammonium sulfate fractionation, alumina C-gamma gel and column chromatography on Sephadex G-200 | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Ovis aries | - |
| kidney | - |
Ovis aries | - |
| liver | - |
Ovis aries | - |
| lung | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.571 | - |
- |
Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoaldehydes + NAD+ + H2O | enzyme from sheep or rat liver also active with NADP+, see also EC 1.2.1.49 | Ovis aries | 2-oxo acids + NADH | - |
? | |
| methylglyoxal + NAD+ + H2O | ir | Ovis aries | pyruvate + NADH + H+ | - |
? | |
| methylglyoxal + NAD+ + H2O | i.e. pyruvaldehyde | Ovis aries | pyruvate + NADH + H+ | - |
? | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
not active below | Ovis aries |
| 9.4 | - |
activity increases up to, Tris buffer | Ovis aries |
| 10.5 | - |
activity increases up to, carbonate buffer | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 3-acetyl-pyridine adenine dinucleotide and thionicotinamide adenine dinucleotide are also effective oxidants | Ovis aries | |
| NAD+ | - |
Ovis aries | |
| NADP+ | enzyme from sheep or rat liver, see also EC 1.2.1.49 | Ovis aries | |
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Release 2023.1 (January 2023)
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