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Literature summary for 1.2.1.26 extracted from

  • Watanabe, S.; Yamada, M.; Ohtsu, I.; Makino, K.
    alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution (2007), J. Biol. Chem., 282, 6685-6695.
    View publication on PubMed
Search for more literature for 1.2.1.26

Activating Compound

Activating Compound Comment Organism Structure
D-galactarate induces KGSADH-II Azospirillum brasilense
D-glucarate induces KGSADH-II Azospirillum brasilense
hydroxy-L-proline induces KGSADH-III Azospirillum brasilense
L-arabinose induces KGSADH-I Azospirillum brasilense

Application

Application Comment Organism
additional information the ALDH branch including ycbD protein is designated the KGSADH subclass (type III) Bacillus subtilis
additional information three different types of KGSADH appear in the bacterial evolutional stage convergently Azospirillum brasilense

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His6-tagged ycbD protein Bacillus subtilis
KGSADH-II overexpressed as His6-tagged protein in Escherichia coli DH5alpha Azospirillum brasilense
KGSADH-III overexpressed as His6-tagged protein in Escherichia coli DH5alpha Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Azospirillum brasilense
0.0075
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Azospirillum brasilense
0.011
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Azospirillum brasilense
0.0149
-
 NADP+
-
 Azospirillum brasilense
0.0246
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Azospirillum brasilense
0.0352
-
 Octylaldehyde
-
 Bacillus subtilis
0.0393
-
 heptaldehyde
-
 Bacillus subtilis
0.0622
-
 NADP+
-
 Azospirillum brasilense
0.0814
-
 hexylaldehyde
-
 Bacillus subtilis
0.094
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Bacillus subtilis
0.1169
-
 NAD+
-
 Azospirillum brasilense
0.145
-
 Valeraldehyde
-
 Bacillus subtilis
0.17
-
 butylaldehyde
-
 Bacillus subtilis
0.2599
-
 NAD+ KGSADH-I Azospirillum brasilense
0.5569
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Bacillus subtilis
0.74
-
 NAD+
-
 Bacillus subtilis
1.08
-
 NADP+
-
 Bacillus subtilis
1.136
-
 NAD+
-
 Azospirillum brasilense
1.46
-
 propionaldehyde
-
 Bacillus subtilis
2.427
-
 NADP+ KGSADH-I Azospirillum brasilense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 gel filtration Azospirillum brasilense
54351
-
 2 * 57000 or 2 * 54351, gel filtration Azospirillum brasilense
55185
-
 2 * 57000 or 2 * 55185, gel filtration Azospirillum brasilense
57000
-
 2 * 57000 or 2 * 54351, gel filtration Azospirillum brasilense
57000
-
 2 * 57000 or 2 * 55185, gel filtration Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense Q08IB7 strain ATCC29145
-
Azospirillum brasilense Q08IC0 strain ATCC29145
-
Azospirillum brasilense Q1JUP4 strain ATCC29145
-
Azospirillum brasilense ATCC 29145 Q08IB7 strain ATCC29145
-
Azospirillum brasilense ATCC 29145 Q08IC0 strain ATCC29145
-
Azospirillum brasilense ATCC 29145 Q1JUP4 strain ATCC29145
-
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
KGSADH-II purified on nickel-nitrilotriacetic acid spin column and by gel filtration Azospirillum brasilense
KGSADH-III purified on nickel-nitrilotriacetic acid spin column and by gel filtration Azospirillum brasilense

Storage Stability

Storage Stability Organism
-35°C, buffer E, pH 8.0, 250 mM imidazole Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dioxopentanoate + NAD+ + H2O
-
 Bacillus subtilis 2-oxoglutarate + NADH + H+
-
 r
2,5-dioxopentanoate + NAD+ + H2O
-
 Azospirillum brasilense 2-oxoglutarate + NADH + H+
-
 r
2,5-dioxopentanoate + NAD+ + H2O
-
 Azospirillum brasilense ATCC 29145 2-oxoglutarate + NADH + H+
-
 r
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
 Bacillus subtilis alpha-ketoglutarate + NAD(P)H + H+
-
 ?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
 Azospirillum brasilense alpha-ketoglutarate + NAD(P)H + H+
-
 ?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
 Azospirillum brasilense ATCC 29145 alpha-ketoglutarate + NAD(P)H + H+
-
 ?
butyraldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-butanoate + NAD(P)H + H+
-
 ?
heptaldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-heptanoate + NAD(P)H + H+
-
 ?
hexylaldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-hexanoate + NAD(P)H + H+
-
 ?
octylaldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-octanoate + NAD(P)H + H+
-
 ?
propionaldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis propionate + NAD(P)H + H+
-
 ?
valeraldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-pentanoate + NAD(P)H + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 57000 or 2 * 54351, gel filtration Azospirillum brasilense
dimer 2 * 57000 or 2 * 55185, gel filtration Azospirillum brasilense
tetramer gel filtration Bacillus subtilis
tetramer gel filtration Azospirillum brasilense

Synonyms

Synonyms Comment Organism
alpha-ketoglutaric semialdehyde dehydrogenase
-
 Bacillus subtilis
alpha-ketoglutaric semialdehyde dehydrogenase
-
 Azospirillum brasilense
alphaKGSA dehydrogenase
-
 Bacillus subtilis
alphaKGSA dehydrogenase
-
 Azospirillum brasilense
KGSADH
-
 Bacillus subtilis
KGSADH-I
-
 Azospirillum brasilense
KGSADH-II
-
 Azospirillum brasilense
KGSADH-III
-
 Azospirillum brasilense
ycbD protein
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.74
-
 NAD+
-
 Bacillus subtilis
0.87
-
 heptaldehyde
-
 Bacillus subtilis
0.97
-
 Octylaldehyde
-
 Bacillus subtilis
1.08
-
 NADP+
-
 Bacillus subtilis
1.12
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Bacillus subtilis
1.29
-
 propionaldehyde
-
 Bacillus subtilis
1.35
-
 butylaldehyde
-
 Bacillus subtilis
1.47
-
 hexylaldehyde
-
 Bacillus subtilis
2.1
-
 Valeraldehyde
-
 Bacillus subtilis
2.75
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Bacillus subtilis
9.86
-
 NADP+ KGSADH-I Azospirillum brasilense
10.2
-
 NADP+
-
 Azospirillum brasilense
15.3
-
 NADP+
-
 Azospirillum brasilense
15.4
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Azospirillum brasilense
16.7
-
 NAD+ KGSADH-I Azospirillum brasilense
21.3
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Azospirillum brasilense
23
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Azospirillum brasilense
35.5
-
 NAD+
-
 Azospirillum brasilense
52.3
-
 NAD+
-
 Azospirillum brasilense
53.9
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus subtilis
NAD+
-
 Azospirillum brasilense
NAD+ no significant preference between NAD+ and NADP+ in activity Azospirillum brasilense
NAD+ prefered cofactor of KGSADH-II Azospirillum brasilense
NADP+
-
 Bacillus subtilis
NADP+
-
 Azospirillum brasilense
NADP+ prefered cofactor of KGSADH-III Azospirillum brasilense