Literature summary for 1.2.1.26 extracted from

Aghaie, A.; Lechaplais, C.; Sirven, P.; Tricot, S.; Besnard-Gonnet, M.; Muselet, D.; de Berardinis, V.; Kreimeyer, A.; Gyapay, G.; Salanoubat, M.; Perret, A.
New Insights into the Alternative D-Glucarate Degradation Pathway (2008), J. Biol. Chem., 283, 15638-15646.

Search for more literature for 1.2.1.26

Application

Application	Comment	Organism
degradation	involved in an alternative pathway of D-glucarate metabolism	Acinetobacter baylyi
degradation	involved in an alternative pathway of D-glucarate metabolism	Pseudomonas putida

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas putida
expression in Escherichia coli BL21 (DE3)	Acinetobacter baylyi

Protein Variants

Protein Variants	Comment	Organism
additional information	alpha-KGSA dehydrogenase activity in cell extracts from the mutant DELTAACIAD0131 grown on succinate as the sole carbon source is undetectable	Acinetobacter baylyi

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibitory effect with alpha-ketoglutarate or glutamate	Acinetobacter baylyi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	alpha-Ketoglutaric semialdehyde	-	Pseudomonas putida
0.015	-	NADP+	-	Acinetobacter baylyi
0.1	-	Glutaraldehyde	with NADP+ as cofactor	Acinetobacter baylyi
0.3	-	propanal	with NADP+ as cofactor	Acinetobacter baylyi
0.31	-	Glutaraldehyde	with NAD+ as cofactor	Acinetobacter baylyi
0.45	-	succinate semialdehyde	with NAD+ as cofactor	Acinetobacter baylyi
0.87	-	NAD+	-	Acinetobacter baylyi
1.05	-	propanal	with NAD+ as cofactor	Acinetobacter baylyi
1.66	-	succinate semialdehyde	with NADP+ as cofactor	Acinetobacter baylyi
3.9	-	acetaldehyde	with NADP+ as cofactor	Acinetobacter baylyi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62000	-	2 * 62000, SDS-PAGE	Acinetobacter baylyi

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter baylyi	Q6FFQ0	-	-
Pseudomonas putida	Q88GW5	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration	Acinetobacter baylyi
to homogeneity	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,5-dioxopentanoate + NAD+ + H2O	-	Acinetobacter baylyi	2-oxoglutarate + NADH + H+	-	r
acetaldehyde + NAD(P)+ + H2O	-	Acinetobacter baylyi	acetate + NAD(P)H + H+	-	?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O	-	Acinetobacter baylyi	alpha-ketoglutarate + NAD(P)H + H+	-	?
alpha-ketoglutaric semialdehyde + NADP+ + H2O	-	Pseudomonas putida	alpha-ketoglutarate + NADPH + H+	-	?
glutaraldehyde + NAD(P)+ + H2O	-	Acinetobacter baylyi	glutarate + NAD(P)H + H+	-	?
propanal + NAD(P)+ + H2O	-	Acinetobacter baylyi	propionate + NAD(P)H + H+	-	?
succinate semialdehyde + NAD(P)+ + H2O	-	Acinetobacter baylyi	? + NAD(P)H + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 62000, SDS-PAGE	Acinetobacter baylyi

Synonyms

Synonyms	Comment	Organism
alpha-ketoglutarate semialdehyde dehydrogenase	-	Acinetobacter baylyi
alpha-ketoglutarate semialdehyde dehydrogenase	-	Pseudomonas putida
alpha-KGSA dehydrogenase	-	Acinetobacter baylyi
alpha-KGSA dehydrogenase	-	Pseudomonas putida
alphaKGSADH	-	Acinetobacter baylyi
alphaKGSADH	-	Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.05	-	succinate semialdehyde	with NADP+ as cofactor	Acinetobacter baylyi
0.06	-	succinate semialdehyde	with NAD+ as cofactor	Acinetobacter baylyi
0.41	-	acetaldehyde	with NADP+ as cofactor	Acinetobacter baylyi
0.58	-	propanal	with NADP+ as cofactor	Acinetobacter baylyi
0.65	-	Glutaraldehyde	with NADP+ as cofactor	Acinetobacter baylyi
2.07	-	propanal	with NAD+ as cofactor	Acinetobacter baylyi
4.13	-	Glutaraldehyde	with NAD+ as cofactor	Acinetobacter baylyi
55	-	NADP+	-	Acinetobacter baylyi
57	-	NAD+	-	Acinetobacter baylyi
197	-	alpha-Ketoglutaric semialdehyde	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Acinetobacter baylyi
NADP+	-	Pseudomonas putida
NADP+	alpha-KGSA dehydrogenase is an NADP+-preferring enzyme, since it is 58times more efficient with NADP+ than with NAD+	Acinetobacter baylyi

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Release 2023.1 (January 2023)