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Literature summary for 1.2.1.27 extracted from

  • Goodwin, G.W.; Rougraff, P.M.; Davis, E.J.; Harris, R.A.
    Purification and characterization of methylmalonate-semialdehyde dehydrogenase from rat liver. Identity to malonate-semialdehyde dehydrogenase (1989), J. Biol. Chem., 264, 14965-14971.
    View publication on PubMed
Search for more literature for 1.2.1.27

Inhibitors

Inhibitors Comment Organism Structure
NADH competitive with NAD+ Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 malonate semialdehyde
-
 Rattus norvegicus
0.0053
-
 2-Methyl-3-oxopropanoate
-
 Rattus norvegicus
0.03
-
 CoA
-
 Rattus norvegicus
0.15
-
 NAD+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 4 * 58000, SDS-PAGE Rattus norvegicus
250000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl-3-oxopropanoate + CoA + NAD+ Rattus norvegicus
-
 propanoyl-CoA + CO2 + NADH
-
 ir
malonate semialdehyde + CoA + NAD+ Rattus norvegicus
-
 acetyl-CoA + CO2 + NADH
-
 ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.83
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
on ice, 1 mM NAD+, 20 h, 77% activity remains Rattus norvegicus
on ice, 10% glycerol, 20 h, 32% activity remains Rattus norvegicus
on ice, 20 h, 11% activity remains Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-3-oxopropanoate + CoA + NAD+
-
 Rattus norvegicus propanoyl-CoA + CO2 + NADH
-
 ir
2-methyl-3-oxopropanoate + CoA + NAD+ both stereoisomers are oxidized Rattus norvegicus propanoyl-CoA + CO2 + NADH
-
 ir
2-methylbutyraldehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
acetaldehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
butyraldehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
isobutyraldehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
malonate semialdehyde + CoA + NAD+
-
 Rattus norvegicus acetyl-CoA + CO2 + NADH
-
 ir
malondialdehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
propanal + NAD+
-
 Rattus norvegicus ? + NADH
-
 ?
succinate semialdehyde + CoA + NAD+ very low activity Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 58000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 2-methyl-3-oxopropanoate + CoA + NAD+ Rattus norvegicus
9
-
 propanal + NAD+ Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 methylmalonate semialdehyde oxidation, less than 90% of maximal activity above and below Rattus norvegicus
8 9.5 propanal oxidation, less than 50% of maximal activity above and below Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
CoA
-
 Rattus norvegicus
NAD+
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0031
-
 NADH
-
 Rattus norvegicus