Cloned (Comment) | Organism |
---|---|
mutant enzymes expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E487K | mutant enzyme shows altered kinetic properties when compared to wild-type enzyme, The Km-value for NAD+ at pH 7.4 increases more than 150fold, the turnover-number decreases 2-10fold, many oriental people posses this variant of liver mitochondrial aldehyde dehydrogenase and have very low levels of enzyme activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
propanal | native enzyme, pH 7.4 | Rattus norvegicus | |
0.0001 | - |
propanal | native enzyme, pH 9.0 | Rattus norvegicus | |
0.0002 | - |
propanal | mutant enzyme E487Q, pH 7.4 | Homo sapiens | |
0.0006 | - |
propanal | mutant enzyme E487K, pH 7.4 | Rattus norvegicus | |
0.0007 | - |
propanal | - |
Rattus norvegicus | |
0.0007 | - |
propanal | native enzyme, pH 7.4 | Homo sapiens | |
0.0014 | - |
propanal | mutant enzyme E487K, pH 7.4 | Homo sapiens | |
0.0017 | - |
propanal | mutant enzyme E487K, pH 9.0 | Rattus norvegicus | |
0.004 | - |
NAD+ | - |
Homo sapiens | |
0.004 | - |
NAD+ | native enzyme, pH 7.4 | Rattus norvegicus | |
0.07 | - |
NAD+ | native enzyme, pH 7.4 | Homo sapiens | |
0.074 | - |
NAD+ | native enzyme, pH 9.0 | Rattus norvegicus | |
0.09 | - |
NAD+ | mutant enzyme E487Q | Homo sapiens | |
0.7 | - |
NAD+ | mutant enzyme E487K, pH 7.4 | Rattus norvegicus | |
1.1 | - |
NAD+ | mutant enzyme E487K, pH 9.0 | Rattus norvegicus | |
7.4 | - |
NAD+ | mutant enzyme E487K | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | - |
Rattus norvegicus | acetate + NADH + H+ | - |
? | |
chloroacetaldehyde + NAD+ + H2O | activity is twice as fast as with acetaldehyde, native enzyme and mutant enzyme E487K | Homo sapiens | chloroacetate + NADH + H+ | - |
? | |
chloroacetaldehyde + NAD+ + H2O | activity is twice as fast as with acetaldehyde, native enzyme and mutant enzyme E487K | Rattus norvegicus | chloroacetate + NADH + H+ | - |
? | |
propanal + NAD+ + H2O | - |
Homo sapiens | propionate + NADH | - |
? | |
propanal + NAD+ + H2O | - |
Rattus norvegicus | propionate + NADH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
additional information | - |
additional information | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Rattus norvegicus |