Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 100 mM, full activation | Rattus norvegicus | |
dithiothreitol | 4 mM, full activation | Rattus norvegicus | |
GSH | 30% activation | Rattus norvegicus |
General Stability | Organism |
---|---|
freezing causes loss of activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzaldehyde | substrate inhibition | Rattus norvegicus | |
Butanal | substrate inhibition | Rattus norvegicus | |
Ca2+ | 0.4 mM, 50% inhibition | Rattus norvegicus | |
D-glyceraldehyde | substrate inhibition | Rattus norvegicus | |
hexanal | substrate inhibition | Rattus norvegicus | |
Isobutanal | substrate inhibition | Rattus norvegicus | |
Mg2+ | 0.4 mM, 50% inhibition | Rattus norvegicus | |
pentanal | substrate inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
hexanal | - |
Rattus norvegicus | |
0.006 | - |
benzaldehyde | - |
Rattus norvegicus | |
0.006 | - |
pentanal | - |
Rattus norvegicus | |
0.007 | - |
Butanal | - |
Rattus norvegicus | |
0.023 | - |
propanal | - |
Rattus norvegicus | |
0.03 | - |
Isobutanal | - |
Rattus norvegicus | |
0.05 | - |
NAD+ | - |
Rattus norvegicus | |
0.246 | - |
acetaldehyde | - |
Rattus norvegicus | |
1.136 | - |
D-glyceraldehyde | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
4°C, phosphate buffer pH 7.4, containing 4 mM dithiothreitol or 10 mM 2-mercaptoethanol, stable for 8-10 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Rattus norvegicus | acetate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Rattus norvegicus | benzoate + NADH + H+ | - |
? | |
butanal + NAD+ + H2O | - |
Rattus norvegicus | butyrate + NADH + H+ | - |
? | |
D-glyceraldehyde + NAD+ + H2O | - |
Rattus norvegicus | D-glycerate + NADH + H+ | - |
r | |
glyceraldehyde + NAD+ + H2O | - |
Rattus norvegicus | glycerate + NADH | - |
? | |
hexanal + NAD+ + H2O | - |
Rattus norvegicus | hexanoate + NADH + H+ | - |
? | |
isobutanal + NAD+ + H2O | - |
Rattus norvegicus | isobutyrate + NADH | - |
? | |
pentanal + NAD+ + H2O | - |
Rattus norvegicus | pentanoate + NADH | - |
? | |
propanal + NAD+ + H2O | - |
Rattus norvegicus | propionate + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
90 min, pH 7.4, 0.2 M phosphate, 4 mM dithiothreitol, stable | Rattus norvegicus |
55 | - |
30 min, 20% loss of activity of diluted solution, 70% loss of activity of concentrated preparations | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 9.5 | 50% of maximal activity at pH 7.3 and at pH 9.5 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
hexanal | - |
Rattus norvegicus | |
0.37 | - |
benzaldehyde | - |
Rattus norvegicus | |
0.54 | - |
pentanal | - |
Rattus norvegicus | |
4.35 | - |
Butanal | - |
Rattus norvegicus | |
14.32 | - |
D-glyceraldehyde | - |
Rattus norvegicus | |
100.1 | - |
Isobutanal | - |
Rattus norvegicus |