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Literature summary for 1.2.1.3 extracted from

  • Ikawa, M.; Impraim, C.C.; Wang, G.; Yoshida, A.
    Isolation and characterization of aldehyde dehydrogenase isozymes from usual and atypical human livers (1983), J. Biol. Chem., 258, 6282-6287.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
acetaldehyde isoenzyme ALDH2 from normal liver Homo sapiens
0.005
-
NAD+ isoenzyme ALDH1 Homo sapiens
0.016
-
NAD+ isoenzyme ALDH2 Homo sapiens
0.022
-
acetaldehyde isoenzyme ALDH1 from normal liver Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoenzyme ALDH1 Homo sapiens 5829
-
mitochondrion isoenzyme ALDH2 Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52600
-
4 * 52600, isoenzyme ALDH1, SDS-PAGE Homo sapiens
52600
-
4 * 52600, isoenzyme ALDH2, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
usual livers contains cytosolic ALDH1 and ALDH2, the atypical phenotype contains only the ALDH1 isoenzyme and immunologically cross-reactive material resulting from a mutation of the ALDH2 locus. 50% of orientals are atypical in respect to ALDH isoenzymes
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme ALDH1 and ALDH2 from normal livers, ALDH1 from the atypical phenotype Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
isoenzyme ALDH1 from atypical liver Homo sapiens
0.51
-
ALDH1 from usual human liver Homo sapiens
0.79
-
ALDH2 from usual human liver Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 10 mM phosphate buffer, pH 6.8, 1 mM EDTA, 0.1% v/v 2-mercaptoethanol, stable for a week Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Homo sapiens acetate + NADH + H+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 52600, isoenzyme ALDH1, SDS-PAGE Homo sapiens
tetramer 4 * 52600, isoenzyme ALDH2, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10.5 pH 8.5: about 40% of the activity of isoenzyme ALDH1 and 2, pH 10.5: about 50% of the activity of isoenzyme ALDH2, about 65% of the activity of isoenzyme ALDH1 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens