KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
acetaldehyde | isoenzyme ALDH2 from normal liver | Homo sapiens | |
0.005 | - |
NAD+ | isoenzyme ALDH1 | Homo sapiens | |
0.016 | - |
NAD+ | isoenzyme ALDH2 | Homo sapiens | |
0.022 | - |
acetaldehyde | isoenzyme ALDH1 from normal liver | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isoenzyme ALDH1 | Homo sapiens | 5829 | - |
mitochondrion | isoenzyme ALDH2 | Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52600 | - |
4 * 52600, isoenzyme ALDH1, SDS-PAGE | Homo sapiens |
52600 | - |
4 * 52600, isoenzyme ALDH2, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
usual livers contains cytosolic ALDH1 and ALDH2, the atypical phenotype contains only the ALDH1 isoenzyme and immunologically cross-reactive material resulting from a mutation of the ALDH2 locus. 50% of orientals are atypical in respect to ALDH isoenzymes | - |
Purification (Comment) | Organism |
---|---|
isoenzyme ALDH1 and ALDH2 from normal livers, ALDH1 from the atypical phenotype | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
isoenzyme ALDH1 from atypical liver | Homo sapiens |
0.51 | - |
ALDH1 from usual human liver | Homo sapiens |
0.79 | - |
ALDH2 from usual human liver | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 10 mM phosphate buffer, pH 6.8, 1 mM EDTA, 0.1% v/v 2-mercaptoethanol, stable for a week | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 52600, isoenzyme ALDH1, SDS-PAGE | Homo sapiens |
tetramer | 4 * 52600, isoenzyme ALDH2, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10.5 | pH 8.5: about 40% of the activity of isoenzyme ALDH1 and 2, pH 10.5: about 50% of the activity of isoenzyme ALDH2, about 65% of the activity of isoenzyme ALDH1 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |