Cloned (Comment) | Organism |
---|---|
when expressed in Escherichia coli M15 most of the protein is localised in inclusion bodies, not used for the enzyme assays | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-propanol | 7% inhibition at 1 mM | Rhodococcus erythropolis | |
EDTA | 10-20% inhibition at 1 mM | Rhodococcus erythropolis | |
iodacetamide | 10-20% inhibition at 1 mM | Rhodococcus erythropolis | |
KCN | 10-20% inhibition at 1 mM | Rhodococcus erythropolis | |
methanol | 17% inhibition at 1 mM | Rhodococcus erythropolis | |
Ni2+ | 52% inhibition at 1 mM | Rhodococcus erythropolis | |
p-hydroxymercuribenzoate | complete inhibition at 0.1 mM | Rhodococcus erythropolis | |
Zn2+ | 73% inhibition at 1 mM | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
octanal | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
0.0018 | - |
hexanal | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
0.0038 | - |
Butanal | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
0.0091 | - |
propanal | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
0.09 | - |
NAD+ | 0,05 mM propanal, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
0.333 | - |
formaldehyde | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 23% activation at 1 mM | Rhodococcus erythropolis | |
NH4+ | 13% activation at 1 mM | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16200 | - |
non-denaturing PAGE | Rhodococcus erythropolis |
54000 | - |
3 * 54000, non-denaturing PAGE | Rhodococcus erythropolis |
54680 | - |
MALDI-TOF MS | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | Q4F895 | - |
- |
Rhodococcus erythropolis UPV-1 | Q4F895 | - |
- |
Purification (Comment) | Organism |
---|---|
two chromatography steps to homogeneity | Rhodococcus erythropolis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rhodococcus erythropolis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.5 | - |
- |
Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanal + NAD+ + H2O | - |
Rhodococcus erythropolis | butyrate + NADH + H+ | - |
? | |
butanal + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | butyrate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Rhodococcus erythropolis | formate + NADH | - |
? | |
formaldehyde + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | formate + NADH | - |
? | |
hexanal + NAD+ + H2O | - |
Rhodococcus erythropolis | hexanoate + NADH + H+ | - |
? | |
hexanal + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | hexanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | - |
Rhodococcus erythropolis | octanoate + NADH | - |
? | |
octanal + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | octanoate + NADH | - |
? | |
propanal + NAD+ + H2O | - |
Rhodococcus erythropolis | propanoate + NADH + H+ | - |
? | |
propanal + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | propanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 54000, non-denaturing PAGE | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase | - |
Rhodococcus erythropolis |
ALDH | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47.5 | - |
with n-propanal as substrate | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
fully stable after 10 min | Rhodococcus erythropolis |
40 | - |
75% activity after 10 min in presence or absence of substrates | Rhodococcus erythropolis |
40 | - |
fully active after 10 min when incubated with NAD+ | Rhodococcus erythropolis |
60 | - |
completely inactive after 10 min | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
with n-propanal as substrate | Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | 60% activity after 16 h at 4°C | Rhodococcus erythropolis |
9.5 | - |
10% activity when stored at a pH above 9.5 | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | totally inactive with NADP+ | Rhodococcus erythropolis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodococcus erythropolis | two-dimensional electrophoresis | - |
5.3 |