Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with 100 mM ACES, pH 6.6-6.8, 5-10 mM MgCl2, 100 mM guanidine HCl, 15% (w/v) PEG 6000, and 4-8 mM dithiothreitol | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K487E | reduced activity | Homo sapiens |
R475Q | the mutant is less thermally stable by 15°C and shows 2fold reduction in kcat-value compared to the wild type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P05091 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALDH2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |