Application | Comment | Organism |
---|---|---|
synthesis | by combining the carboxylic acid reductase-dependent pathway with an exogenous fatty acid-generating lipase, natural oils (coconut oil, palm oil, and algal oil bodies) can be enzymatically converted into fatty alcohols across a broad chain length range (C8-C18) | Mycobacterium marinum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium marinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
NADPH | pH and temperature not specified in the publication | Mycobacterium marinum | |
0.115 | - |
ATP | pH and temperature not specified in the publication | Mycobacterium marinum | |
0.362 | - |
benzoic acid | pH and temperature not specified in the publication | Mycobacterium marinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fatty acid + ATP + NADPH + H+ | Mycobacterium marinum | - |
fatty aldehyde + AMP + diphosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium marinum | B2HN69 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Mycobacterium marinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoic acid + ATP + NADPH + H+ | highest turnover number | Mycobacterium marinum | benzaldehyde + AMP + diphosphate + NADP+ | - |
? | |
butyric acid + ATP + NADPH + H+ | - |
Mycobacterium marinum | butyraldehyde + AMP + diphosphate + NADP+ | highest Km value | ? | |
capric acid + ATP + NADPH + H+ | - |
Mycobacterium marinum | capraldehyde + AMP + diphosphate + NADP+ | - |
? | |
caproic acid + ATP + NADPH + H+ | - |
Mycobacterium marinum | caproaldehyde + AMP + diphosphate + NADP+ | - |
? | |
caprylic acid + ATP + NADPH + H+ | - |
Mycobacterium marinum | caprylaldehyde + AMP + diphosphate + NADP+ | - |
? | |
fatty acid + ATP + NADPH + H+ | - |
Mycobacterium marinum | fatty aldehyde + AMP + diphosphate + NADP+ | - |
? | |
lauric acid + ATP + NADPH + H+ | highest catalytic efficiency | Mycobacterium marinum | lauraldehyde + AMP + diphosphate + NADP+ | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | 37 | in vitro half-lives of 73, 70, and 48 h at 26, 30, and 37°C | Mycobacterium marinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Mycobacterium marinum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | the enzyme has a selective preference for NADPH over NADH | Mycobacterium marinum |