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Literature summary for 1.2.1.30 extracted from

  • Akhtar, M.K.; Turner, N.J.; Jones, P.R.
    Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities (2013), Proc. Natl. Acad. Sci. USA, 110, 87-92.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis by combining the carboxylic acid reductase-dependent pathway with an exogenous fatty acid-generating lipase, natural oils (coconut oil, palm oil, and algal oil bodies) can be enzymatically converted into fatty alcohols across a broad chain length range (C8-C18) Mycobacterium marinum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium marinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
NADPH pH and temperature not specified in the publication Mycobacterium marinum
0.115
-
ATP pH and temperature not specified in the publication Mycobacterium marinum
0.362
-
benzoic acid pH and temperature not specified in the publication Mycobacterium marinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fatty acid + ATP + NADPH + H+ Mycobacterium marinum
-
fatty aldehyde + AMP + diphosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium marinum B2HN69
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Mycobacterium marinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoic acid + ATP + NADPH + H+ highest turnover number Mycobacterium marinum benzaldehyde + AMP + diphosphate + NADP+
-
?
butyric acid + ATP + NADPH + H+
-
Mycobacterium marinum butyraldehyde + AMP + diphosphate + NADP+ highest Km value ?
capric acid + ATP + NADPH + H+
-
Mycobacterium marinum capraldehyde + AMP + diphosphate + NADP+
-
?
caproic acid + ATP + NADPH + H+
-
Mycobacterium marinum caproaldehyde + AMP + diphosphate + NADP+
-
?
caprylic acid + ATP + NADPH + H+
-
Mycobacterium marinum caprylaldehyde + AMP + diphosphate + NADP+
-
?
fatty acid + ATP + NADPH + H+
-
Mycobacterium marinum fatty aldehyde + AMP + diphosphate + NADP+
-
?
lauric acid + ATP + NADPH + H+ highest catalytic efficiency Mycobacterium marinum lauraldehyde + AMP + diphosphate + NADP+
-
?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
26 37 in vitro half-lives of 73, 70, and 48 h at 26, 30, and 37°C Mycobacterium marinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Mycobacterium marinum

Cofactor

Cofactor Comment Organism Structure
NADPH the enzyme has a selective preference for NADPH over NADH Mycobacterium marinum