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Literature summary for 1.2.1.32 extracted from
- Purification and characterisation of TOL plasmid-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase of Pseudomonas putida (1990), Eur. J. Biochem., 191, 705-714.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | equimolar amount reduces benzaldehyde oxidation activity by 44% | Pseudomonas putida |  |
| N-ethylmaleimide | equimolar amount inactivates benzaldehyde oxidation completely, 5 mM 2-mercaptoethanol restores activity | Pseudomonas putida | |
| p-chloromercuribenzoate | equimolar amount inactivates benzaldehyde oxidation completely, 5 mM 2-mercaptoethanol restores activity | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
benzaldehyde | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, SDS-PAGE | Pseudomonas putida |
| 126000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
carrying the TOL plasmid pWW0 | - |
| Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | - |
carrying the TOL plasmid pWW0 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| erroneously identified as benzaldehyde dehydrogenase | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-chlorobenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-chlorobenzoate + NADH | - |
? | |
| 3-methylbenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-methylbenzoate + NADH | - |
? | |
| 3-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-nitrobenzoate + NADH | - |
? | |
| 4-carboxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-carboxybenzoate + NADH | - |
? | |
| 4-methoxybenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 4-methoxybenzoate + NADH + H+ | - |
? | |
| 4-methylbenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 4-methylbenzoate + NADH | - |
? | |
| benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | benzoate + NADH + H+ | - |
? | |
| additional information | no reaction with 2-methylbenzaldehyde | Pseudomonas putida | ? | - |
? | |
| additional information | no reaction with 2-hydroxybenzaldehyde, 3-hydroxybenzaldehyde | Pseudomonas putida | ? | - |
? | |
| pyridine 4-carboxyaldehyde + NAD+ + H2O | - |
Pseudomonas putida | pyridine 4-carboxylate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, SDS-PAGE | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
benzaldehyde oxidation | Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | benzaldehyde oxidation, more than 50% of maximal activity between pH 8 and 11 | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas putida | |
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