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Literature summary for 1.2.1.39 extracted from

  • Hirano, J.; Miyamoto, K.; Ohta, H.
    Purification and characterization of aldehyde dehydrogenase with a broad substrate specificity originated from 2-phenylethanol-assimilating Brevibacterium sp. KU1309 (2007), Appl. Microbiol. Biotechnol., 76, 357-363.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
8-Quinolinol 34% inhibition in the presence of 1 mM Brevibacterium sp.
Ba2+ 19% inhibition in the presence of 1 mM Brevibacterium sp.
Cu2+ 44% inhibition in the presence of 1 mM Brevibacterium sp.
EDTA 18% inhibition in the presence of 1 mM Brevibacterium sp.
Hg2+ 77% inhibition in the presence of 1 mM Brevibacterium sp.
hydrozine 51% inhibition in the presence of 1 mM Brevibacterium sp.
iodoacetamide completely inhibits in the presence of 1 mM Brevibacterium sp.
iodoacetate 18% inhibition in the presence of 1 mM Brevibacterium sp.
N-ethylmaleinimide completely inhibits in the presence of 1 mM Brevibacterium sp.
NaN3 25% inhibition in the presence of 1 mM Brevibacterium sp.
Ni2+ 12% inhibition in the presence of 1 mM Brevibacterium sp.
p-chloromercuribenzoate completely inhibits in the presence of 1 mM Brevibacterium sp.
phenylacetaldehyde high concentration (0.1 mM) inhibits Brevibacterium sp.
Zn2+ 41% inhibition in the presence of 1 mM Brevibacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00124
-
phenylacetaldehyde
-
Brevibacterium sp.
0.116
-
NAD+
-
Brevibacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Brevibacterium sp. 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM stimulates by 53% Brevibacterium sp.
Mg2+ 1 mM stimulates by 42% Brevibacterium sp.
Mn2+ 1 mM stimulates by 17% Brevibacterium sp.
additional information is not stimulated by 10 mM KCl Brevibacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
4 * 61000, SDS-PAGE Brevibacterium sp.
219000
-
gel filtration Brevibacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetaldehyde + NAD+ + H2O Brevibacterium sp.
-
phenylacetate + NADH + H+
-
?
phenylacetaldehyde + NAD+ + H2O Brevibacterium sp. KU1309
-
phenylacetate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp. KU1309
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, 15.9fold with a 0.21% yield Brevibacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.261
-
cell-free extract Brevibacterium sp.
4.16
-
15.9fold purified enzyme Brevibacterium sp.

Storage Stability

Storage Stability Organism
4°C, 10 mM Tris-HCl buffer Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthaldehyde + NAD+ + H2O 24% activity relative to phenylacetaldehyde Brevibacterium sp. 1-naphthoate + NADH + H+
-
?
3-phenylpropionaldehyde + NAD+ + H2O 34% activity relative to phenylacetaldehyde Brevibacterium sp. 3-phenylpropionate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O 54% activity relative to phenylacetaldehyde Brevibacterium sp. benzoate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O 54% activity relative to phenylacetaldehyde Brevibacterium sp. KU1309 benzoate + NADH + H+
-
?
decanal + NAD+ + H2O 17% activity relative to phenylacetaldehyde Brevibacterium sp. decanoate + NADH + H+
-
?
hexanal + NAD+ + H2O 21% activity relative to phenylacetaldehyde Brevibacterium sp. hexanoate + NADH + H+
-
?
hexanal + NAD+ + H2O 21% activity relative to phenylacetaldehyde Brevibacterium sp. KU1309 hexanoate + NADH + H+
-
?
octanal + NAD+ + H2O 31% activity relative to phenylacetaldehyde Brevibacterium sp. octanoate + NADH + H+
-
?
octanal + NAD+ + H2O 31% activity relative to phenylacetaldehyde Brevibacterium sp. KU1309 octanoate + NADH + H+
-
?
phenylacetaldehyde + NAD+ + H2O
-
Brevibacterium sp. phenylacetate + NADH + H+
-
?
phenylacetaldehyde + NAD+ + H2O best substrate Brevibacterium sp. phenylacetate + NADH + H+
-
?
phenylacetaldehyde + NAD+ + H2O
-
Brevibacterium sp. KU1309 phenylacetate + NADH + H+
-
?
phenylacetaldehyde + NAD+ + H2O best substrate Brevibacterium sp. KU1309 phenylacetate + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 61000, SDS-PAGE Brevibacterium sp.

Synonyms

Synonyms Comment Organism
PADH
-
Brevibacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Brevibacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Brevibacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Brevibacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 at pH 8.0, PADH retains 60% of the initial activity Brevibacterium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Brevibacterium sp.