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Literature summary for 1.2.1.4 extracted from

  • Hommel, R.; Kurth, J.; Kleber, H.P.
    NADP+-dependent aldehyde dehydrogenase from Acetobacter rancens CCM 1774: purification and properties (1988), J. Basic Microbiol., 28, 25-33.
No PubMed abstract available
Search for more literature for 1.2.1.4

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Acetobacter pasteurianus
EDTA 0.5 mM, 50% inhibition Acetobacter pasteurianus
ethyl acetate
-
 Acetobacter pasteurianus
Fe2+ 1 mM ZnCl2, complete inhibition, irreversible Acetobacter pasteurianus
Hg2+ 0.5 mM HgCl2, complete inhibition, irreversible Acetobacter pasteurianus
NADPH product inhibition Acetobacter pasteurianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 NADP+ in presence of propanal Acetobacter pasteurianus
0.031
-
 acetaldehyde
-
 Acetobacter pasteurianus
0.15
-
 propanal
-
 Acetobacter pasteurianus
0.38
-
 formaldehyde
-
 Acetobacter pasteurianus
0.98
-
 Butanal
-
 Acetobacter pasteurianus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Acetobacter pasteurianus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 gel filtration Acetobacter pasteurianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Acetobacter pasteurianus the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids ?
-
 ?
additional information Acetobacter pasteurianus CCM 1774 the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acetobacter pasteurianus
-
 CCM 1774
-
Acetobacter pasteurianus CCM 1774
-
 CCM 1774
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acetobacter pasteurianus

Reaction

Reaction Comment Organism Reaction ID
an aldehyde + NADP+ + H2O = a carboxylate + NADPH + H+ random kinetic mechanism Acetobacter pasteurianus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Acetobacter pasteurianus

Storage Stability

Storage Stability Organism
4°C, phosphate buffer, pH 6.8-7.5, half-life: 5 months Acetobacter pasteurianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADP+ + H2O
-
 Acetobacter pasteurianus acetate + NADPH + H+
-
 ?
acetaldehyde + NADP+ + H2O
-
 Acetobacter pasteurianus CCM 1774 acetate + NADPH + H+
-
 ?
butanal + NADP+ + H2O
-
 Acetobacter pasteurianus butyrate + NADPH + H+
-
 ?
butanal + NADP+ + H2O
-
 Acetobacter pasteurianus CCM 1774 butyrate + NADPH + H+
-
 ?
formaldehyde + NADP+ + H2O low activity Acetobacter pasteurianus formate + NADPH + H+
-
 ?
formaldehyde + NADP+ + H2O low activity Acetobacter pasteurianus CCM 1774 formate + NADPH + H+
-
 ?
additional information the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids Acetobacter pasteurianus ?
-
 ?
additional information the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids Acetobacter pasteurianus CCM 1774 ?
-
 ?
propanal + NADP+ + H2O
-
 Acetobacter pasteurianus propionate + NADPH + H+
-
 ?
propanal + NADP+ + H2O
-
 Acetobacter pasteurianus CCM 1774 propionate + NADPH + H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Acetobacter pasteurianus

pH Range

pH Minimum pH Maximum Comment Organism
5 9.2 pH 5.0: 10% of maximal activity, pH 9.2: 15% of maximal activity Acetobacter pasteurianus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9.5 30 min, stable Acetobacter pasteurianus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Acetobacter pasteurianus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.043
-
 NADPH
-
 Acetobacter pasteurianus