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Literature summary for 1.2.1.4 extracted from

  • Ho, K.K.; Weiner, H.
    Isolation and characterization of an aldehyde dehydrogenase encoded by the aldB gene of Escherichia coli (2005), J. Bacteriol., 187, 1067-1073.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
ethanol 0.5-2% increases activity in crude extracts Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
native and mutant protein expressed in Escherichia coli DH5alpha Escherichia coli

Protein Variants

Protein Variants Comment Organism
R197E very low activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 acetaldehyde pH 7.5, 25°C Escherichia coli
0.0036
-
 Chloroacetaldehyde pH 7.5, 25°C Escherichia coli
0.0058
-
 propanal pH 7.5, 25°C Escherichia coli
0.056
-
 benzaldehyde pH 7.5, 25°C Escherichia coli
0.1
-
 NADP+ pH 7.5, 25°C Escherichia coli
0.9
-
 4-hydroperoxycyclophosphamide pH 7.5, 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates activity by about 100% Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56352
-
 4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry Escherichia coli
58000
-
 4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry Escherichia coli
220000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + NADP+ + H2O Escherichia coli
-
 acetate + NADPH + H+
-
 ?
an aldehyde + NADP+ + H2O Escherichia coli removal of aldehydes and alcohols in cells under stress an acid + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strain ATCC 700926D, highest activity in cells grown with ethanol
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
 purified enzyme, pH 7.5, 25°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroperoxycyclophosphamide + NADP+ low activity Escherichia coli ?
-
 ?
acetaldehyde + NADP+ + H2O
-
 Escherichia coli acetate + NADPH + H+
-
 ?
an aldehyde + NADP+ + H2O removal of aldehydes and alcohols in cells under stress Escherichia coli an acid + NADPH + H+
-
 ?
benzaldehyde + NADP+ l40% of the activity with acetaldehyde Escherichia coli benzoate + NADPH
-
 ?
chloroacetaldehyde + NADP+ + H2O most effective substrate in the absence of Mg2+ Escherichia coli chloroacetate + NADPH + H+
-
 ?
mafosfamide + NADP+ low activity Escherichia coli ?
-
 ?
additional information no activity with glycolaldehyde and glyceraldehyde Escherichia coli ?
-
 ?
propanal + NADP+ + H2O much lower activity than with acetaldehyde Escherichia coli propionate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NAD+ Escherichia coli
NADP+
-
 Escherichia coli