General Stability | Organism |
---|---|
2-mercaptoethanol: stabilizes , only stable in presence of | Glycine max |
ethyleneglycol, 15%, stabilizes | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 0.01 mM, 50% inhibition | Glycine max | |
CoASH | product inhibition | Glycine max | |
iodoacetamide | 1 mM, 50% inhibition | Glycine max | |
NADP+ | product inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NADPH | cosubstrate feruloyl-CoA | Glycine max | |
0.05 | - |
5-hydroxyferuloyl-CoA | - |
Glycine max | |
0.073 | - |
feruloyl-CoA | - |
Glycine max | |
0.1 | - |
p-Coumaroyl-CoA | - |
Glycine max | |
0.12 | - |
NADPH | cosubstrate sinapoyl-CoA | Glycine max | |
0.2 | - |
caffeoyl-CoA | - |
Glycine max | |
0.29 | - |
NADPH | cosubstrate p-coumaroyl-CoA | Glycine max | |
0.4 | - |
sinapoyl-CoA | - |
Glycine max | |
0.9 | - |
cinnamoyl-CoA | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000 SDS-PAGE | Glycine max |
38000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
L. var. Mandarin, soybean | - |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.2 | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
-20°C, 100 mM Tris-HCl buffer, pH 7.5, 10% ethyleneglycol, several weeks | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyferuloyl-CoA + NADPH + H+ | i.e. 4,5-dihydroxy-3-methoxycinnamoyl-CoA | Glycine max | 5-hydroxyferulic aldehyde + CoA + NADP+ | - |
? | |
caffeoyl-CoA + NADPH | - |
Glycine max | caffeic aldehyde + CoA + NADP+ | - |
? | |
cinnamoyl-CoA + NADPH | - |
Glycine max | cinnamaldehyde + CoA + NADP+ | - |
? | |
feruloyl-CoA + NADPH | - |
Glycine max | ferulic aldehyde + CoA + NADP+ | - |
? | |
p-coumaroyl-CoA + NADPH | - |
Glycine max | p-coumaric aldehyde + CoA + NADP+ | - |
? | |
sinapoyl-CoA + NADPH | - |
Glycine max | sinapic aldehyde + CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000 SDS-PAGE | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.2 | - |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 8 | pH 5.4: about 10% of activity maximum, pH 8.0: about 15% of activity maximum | Glycine max |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
30°C, 30 min, most stable around | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Glycine max |