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Literature summary for 1.2.1.44 extracted from

  • Sonawane, P.; Patel, K.; Vishwakarma, R.K.; Srivastava, S.; Singh, S.; Gaikwad, S.; Khan, B.M.
    Probing the active site of cinnamoyl CoA reductase 1 (Ll-CCRH1) from Leucaena leucocephala (2013), Int. J. Biol. Macromol., 60C, 33-38.
    View publication on PubMed
Search for more literature for 1.2.1.44

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leucaena leucocephala

Protein Variants

Protein Variants Comment Organism
D77G the mutant displays higher Km values (up to 4fold), indicating lower affinity toward substrate, while catalytic efficiencies for these mutant is notably decreased Leucaena leucocephala
F30V the mutant displays higher Km values (up to 4fold), indicating lower affinity toward substrate, while catalytic efficiencies for these mutant is notably decreased Leucaena leucocephala
F30V/I31N the mutant shows approximately 7fold increase in Km and 8fold decrease in kcat/Km values Leucaena leucocephala
H215L the mutant shows no significant change in Km values compared to the wild type enzyme Leucaena leucocephala
I131N the mutant shows slightly reduced catalytic efficiency compared to the wild type Leucaena leucocephala
K174M the mutant shows complete loss of activity with feruloyl-CoA as substrate Leucaena leucocephala
L64W the mutant shows no significant change in Km values compared to the wild type enzyme Leucaena leucocephala
R51G the mutant displays higher Km values (up to 4fold), indicating lower affinity toward substrate, while catalytic efficiencies for these mutant is notably decreased Leucaena leucocephala
R51G/D77G the mutant displays 5fold increase in Km and around 9fold reduction in specificity constant Leucaena leucocephala
S136A the mutant shows complete loss of activity with feruloyl-CoA as substrate Leucaena leucocephala
S212G the mutant exhibits the catalytic efficiencies less than 10% of wild type enzyme Leucaena leucocephala
S212G the mutant shows a 2.5fold increase in Km, 7fold reduction in kcat and 15fold decrease in kcat/Km Leucaena leucocephala
S99G the mutant shows no significant change in Km values compared to the wild type enzyme Leucaena leucocephala
V200E the mutant shows reduced catalytic efficiency compared to the wild type Leucaena leucocephala
Y170H the mutant shows complete loss of activity with feruloyl-CoA as substrate Leucaena leucocephala

Inhibitors

Inhibitors Comment Organism Structure
citraconic anhydride 31% residual activity at 1.5 mM with feruloyl-CoA as substrate Leucaena leucocephala
diethylpyrocarbonate 44% residual activity at 3 mM with feruloyl-CoA as substrate Leucaena leucocephala
additional information not inhibited by N-ethylmaleimide, 4-hydroxymercurybenzoate, and N-bromosuccinimide Leucaena leucocephala
N-acetyl imidazole 50% residual activity at 3 mM with feruloyl-CoA as substrate Leucaena leucocephala
Phenylglyoxal 47% residual activity at 1 mM with feruloyl-CoA as substrate Leucaena leucocephala
phenylmethylsulfonyl fluoride 60% residual activity at 1 mM with feruloyl-CoA as substrate Leucaena leucocephala
Woodward's reagent K 54% residual activity at 0.02 mM with feruloyl-CoA as substrate Leucaena leucocephala

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 feruloyl-CoA mutant enzyme S99G, pH and temperature not specified in the publication Leucaena leucocephala
0.036
-
 feruloyl-CoA wild type enzyme, pH and temperature not specified in the publication Leucaena leucocephala
0.039
-
 feruloyl-CoA mutant enzyme L64W, pH and temperature not specified in the publication Leucaena leucocephala
0.042
-
 feruloyl-CoA mutant enzyme H215L, pH and temperature not specified in the publication Leucaena leucocephala
0.047
-
 feruloyl-CoA mutant enzyme R51G, pH and temperature not specified in the publication Leucaena leucocephala
0.057
-
 feruloyl-CoA mutant enzyme D77G, pH and temperature not specified in the publication Leucaena leucocephala
0.06
-
 feruloyl-CoA mutant enzyme V200E, pH and temperature not specified in the publication Leucaena leucocephala
0.079
-
 feruloyl-CoA mutant enzyme S212G, pH and temperature not specified in the publication Leucaena leucocephala
0.096
-
 feruloyl-CoA mutant enzyme F30V, pH and temperature not specified in the publication Leucaena leucocephala
0.102
-
 feruloyl-CoA mutant enzyme I31N, pH and temperature not specified in the publication Leucaena leucocephala
0.166
-
 feruloyl-CoA mutant enzyme R51G/D77G, pH and temperature not specified in the publication Leucaena leucocephala
0.244
-
 feruloyl-CoA mutant enzyme RF30V/I31N, pH and temperature not specified in the publication Leucaena leucocephala

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl-CoA + NADPH + H+ Leucaena leucocephala
-
 ferulic aldehyde + CoA + NADP+
-
 ?
sinapoyl-CoA + NADPH + H+ Leucaena leucocephala
-
 sinapaldehyde + CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala Q08GL0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feruloyl-CoA + NADPH + H+
-
 Leucaena leucocephala ferulic aldehyde + CoA + NADP+
-
 ?
sinapoyl-CoA + NADPH + H+
-
 Leucaena leucocephala sinapaldehyde + CoA + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
CCRH1
-
 Leucaena leucocephala
cinnamoyl CoA reductase 1
-
 Leucaena leucocephala

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24
-
 feruloyl-CoA mutant enzyme S212G, pH and temperature not specified in the publication Leucaena leucocephala
44
-
 feruloyl-CoA mutant enzyme H215L, pH and temperature not specified in the publication Leucaena leucocephala
84
-
 feruloyl-CoA mutant enzyme R51G/D77G, pH and temperature not specified in the publication Leucaena leucocephala
100
-
 feruloyl-CoA mutant enzyme R51G, pH and temperature not specified in the publication Leucaena leucocephala
108
-
 feruloyl-CoA mutant enzyme D77G, pH and temperature not specified in the publication Leucaena leucocephala
122
-
 feruloyl-CoA mutant enzyme V200E, pH and temperature not specified in the publication Leucaena leucocephala
137
-
 feruloyl-CoA mutant enzyme F30V, pH and temperature not specified in the publication Leucaena leucocephala
142
-
 feruloyl-CoA mutant enzyme RF30V/I31N, pH and temperature not specified in the publication Leucaena leucocephala
144
-
 feruloyl-CoA mutant enzyme S99G, pH and temperature not specified in the publication Leucaena leucocephala
155
-
 feruloyl-CoA mutant enzyme I31N, pH and temperature not specified in the publication Leucaena leucocephala
160
-
 feruloyl-CoA mutant enzyme L64W, pH and temperature not specified in the publication Leucaena leucocephala
162
-
 feruloyl-CoA wild type enzyme, pH and temperature not specified in the publication Leucaena leucocephala

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Leucaena leucocephala

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
310
-
 feruloyl-CoA mutant enzyme S212G, pH and temperature not specified in the publication Leucaena leucocephala
510
-
 feruloyl-CoA mutant enzyme R51G/D77G, pH and temperature not specified in the publication Leucaena leucocephala
590
-
 feruloyl-CoA mutant enzyme RF30V/I31N, pH and temperature not specified in the publication Leucaena leucocephala
1000
-
 feruloyl-CoA mutant enzyme H215L, pH and temperature not specified in the publication Leucaena leucocephala
1400
-
 feruloyl-CoA mutant enzyme F30V, pH and temperature not specified in the publication Leucaena leucocephala
1500
-
 feruloyl-CoA mutant enzyme I31N, pH and temperature not specified in the publication Leucaena leucocephala
1900
-
 feruloyl-CoA mutant enzyme D77G, pH and temperature not specified in the publication Leucaena leucocephala
2000
-
 feruloyl-CoA mutant enzyme V200E, pH and temperature not specified in the publication Leucaena leucocephala
2100
-
 feruloyl-CoA mutant enzyme R51G, pH and temperature not specified in the publication Leucaena leucocephala
4100
-
 feruloyl-CoA mutant enzyme L64W, pH and temperature not specified in the publication Leucaena leucocephala
4500
-
 feruloyl-CoA mutant enzyme S99G, pH and temperature not specified in the publication Leucaena leucocephala
4600
-
 feruloyl-CoA wild type enzyme, pH and temperature not specified in the publication Leucaena leucocephala