General Stability | Organism |
---|---|
freezing and thawing, more than once causes complete loss of activity of enzyme from breast adenocarcinoma cell line, enzyme from stomach mucosa retains 40% of its activity even after being frozen and thawed eight times | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
NAD+ | reaction with benzaldehyde, enzyme from stomach mucosa | Homo sapiens | |
0.067 | - |
octanal | reaction with NAD+ or NADP+, enzyme from breast adenocarcinoma cell line | Homo sapiens | |
0.091 | - |
4-Pyridinecarboxaldehyde | reaction with NAD+ or NADP+, enzyme from breast adenocarcinoma cell line | Homo sapiens | |
0.104 | - |
octanal | reaction with NADP+, enzyme from stomach mucosa | Homo sapiens | |
0.113 | - |
octanal | reaction with NAD+, enzyme from stomach mucosa | Homo sapiens | |
0.19 | - |
4-Pyridinecarboxaldehyde | reaction with NAD+ or NADP+, enzyme from stomach mucosa | Homo sapiens | |
0.486 | - |
benzaldehyde | reaction with NADP+, enzyme from stomach mucosa | Homo sapiens | |
0.505 | - |
benzaldehyde | reaction with NAD+, enzyme from stomach mucosa | Homo sapiens | |
0.55 | - |
NAD+ | reaction with benzaldehyde, enzyme from adenocarcinoma cell line | Homo sapiens | |
0.64 | - |
benzaldehyde | reaction with NAD+ or NADP+, enzyme from breast adenocarcinoma cell line | Homo sapiens | |
0.64 | - |
aldophosphamide | reaction with NAD+ | Homo sapiens | |
0.94 | - |
NADP+ | reaction with benzaldehyde, enzyme from stomach mucosa | Homo sapiens | |
1 | - |
NADP+ | reaction with benzaldehyde, enzyme from adenocarcinoma cell line | Homo sapiens | |
19 | - |
propanal | reaction with NADP+, enzyme from stomach mucosa | Homo sapiens | |
19.06 | - |
propanal | reaction with NAD+, enzyme from stomach mucosa | Homo sapiens | |
80 | - |
acetaldehyde | reaction with NAD+, enzyme from stomach mucosa | Homo sapiens | |
81 | - |
acetaldehyde | reaction with NADP+, enzyme from stomach mucosa | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, enzyme from breast adenocarcinoma cell line, SDS-PAGE | Homo sapiens |
54500 | - |
2 * 54500, enzyme from stomach mucosa, SDS-PAGE | Homo sapiens |
108000 | - |
enzyme from stomach mucosa, gel filtration | Homo sapiens |
110000 | - |
non-denaturing PAGE | Homo sapiens |
125000 | - |
enzyme from breast adenocarcinoma cell line, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast adenocarcinoma cell | exhibiting oxazaphosphorine-specific acquired resistance, enzyme form ALDH3 | Homo sapiens | - |
stomach | mucosa | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.67 | - |
enzyme from breast adenocarcinoma cell line | Homo sapiens |
32.95 | - |
enzyme from stomach mucosa | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, pH 7.5, 25 mM sodium phosphate supplemented with 1 mM EDTA and 0.05% dithiothreitol, 10% glycerol, enzyme from breast adenocarcinoma cell line completely loses activity after 15 days, enzyme from stomach mucosa loses 75% of its activity after 3 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-pyridinecarboxaldehyde + NAD+ + H2O | - |
Homo sapiens | 4-pyridinecarboxylate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | activity with the enzyme from stomach mucosa, no activity with the enzyme from breast adenocarcinoma cell line | Homo sapiens | acetate + NADH + H+ | - |
? | |
aldophosphamide + NAD+ | - |
Homo sapiens | N,N-bis-(2-chloroethyl)-diaminophosphoric-(2-carboxyethylester) + ? | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Homo sapiens | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NADP+ | - |
Homo sapiens | benzoate + NADPH | - |
? | |
octanal + NAD+ + H2O | - |
Homo sapiens | octanoate + NADH + H+ | - |
? | |
propanal + NAD+ + H2O | - |
Homo sapiens | propionate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, enzyme from breast adenocarcinoma cell line, SDS-PAGE | Homo sapiens |
dimer | 2 * 54500, enzyme from stomach mucosa, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | benzaldehyde oxidation, enzyme from breast adenocarcinoma cell line | Homo sapiens |
8 | 9 | benzaldehyde oxidation, enzyme from stomach mucosa | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | highest stability of enzyme from breast adenocarcinoma cell line | Homo sapiens |
7.5 | 9.5 | highest stability of enzyme from stomach mucosa | Homo sapiens |