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Literature summary for 1.2.1.50 extracted from

  • Hofvander, P.; Doan, T.T.; Hamberg, M.
    A prokaryotic acyl-CoA reductase performing reduction of fatty acyl-CoA to fatty alcohol (2011), FEBS Lett., 585, 3538-3543.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
plasmid pHisMBPMarFAR harbouring the sequence of hypothetical protein Maqu_2220 added by His-tag and maltose binding protein transformed to Escherichia coli strain Rosetta (DE3) for heterologous protein expression Marinobacter nauticus

Organism

Organism UniProt Comment Textmining
Marinobacter nauticus
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-
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Purification (Commentary)

Purification (Comment) Organism
via fusion protein with maltose binding protein and an N-terminal His-tag fusion Marinobacter nauticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0273
-
ricinoleoyl-CoA, pH 7.0, 30°C Marinobacter nauticus
0.043
-
palmitoyl-CoA, pH 7.0, 30°C Marinobacter nauticus
0.0677
-
stearoyl-CoA, pH 7.0, 30°C Marinobacter nauticus
0.0774
-
icosanoyl-CoA, pH 7.0, 30°C Marinobacter nauticus
0.1082
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oleoyl-CoA, pH 7.0, 30°C Marinobacter nauticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
erucoyl-CoA + NADPH + H+ erucoyl-CoA i.e. (13Z)-docos-13-enoyl-CoA Marinobacter nauticus (13Z)-docos-13-enal + CoA + NADP+ further direct reduction of (13Z)-docos-13-enal into the corresponding (13Z)-docos-13-en-1-ol ?
icosanoyl-CoA + NADPH + H+
-
Marinobacter nauticus icosanal + CoA + NADP+ further direct reduction of icosanal into the corresponding icosan-1-ol ?
additional information in contrast to other prokaryotes, the enzyme performs the two reduction steps from acyl-CoA to fatty alcohol in a single step which is typical for eukaryotes Marinobacter nauticus ?
-
?
additional information shorter acyl chains than 16 carbon are less effectively utilized and with these substrates substantial amounts of both free fatty acids and fatty aldehyde are formed Marinobacter nauticus ?
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?
oleoyl-CoA + NADPH + H+
-
Marinobacter nauticus (9Z)-octadec-9-enal + CoA + NADP+ further direct reduction of (9Z)-octadec-9-enal into the corresponding (9Z)-octadec-9-en-1-ol ?
palmitoyl-CoA + NADPH + H+
-
Marinobacter nauticus hexadecanal + CoA + NADP+ further direct reduction of hexadecanal into the corresponding hexadecan-1-ol ?
palmitoyl-[acyl-carrier protein] + NADPH + H+
-
Marinobacter nauticus hexadecanal + [acyl-carrier protein] + NADP+ further direct reduction of hexadecanal into the corresponding hexadecan-1-ol ?
ricinoleoyl-CoA + NADPH + H+ ricinoleoyl-CoA i.e. (9Z,12R)-12-hydroxyoctadec-9-enoyl-CoA Marinobacter nauticus (9Z,12R)-12-hydroxyoctadec-9-enal + CoA + NADP+ further direct reduction of (9Z,12R)-12-hydroxyoctadec-9-enal into the corresponding (9Z,12R)-octadec-9-ene-1,12-diol ?
stearoyl-CoA + NADPH + H+
-
Marinobacter nauticus octadecanal + CoA + NADP+ further direct reduction of octadecanal into the corresponding octadecan-1-ol ?