Cloned (Comment) | Organism |
---|---|
plasmid pHisMBPMarFAR harbouring the sequence of hypothetical protein Maqu_2220 added by His-tag and maltose binding protein transformed to Escherichia coli strain Rosetta (DE3) for heterologous protein expression | Marinobacter nauticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marinobacter nauticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
via fusion protein with maltose binding protein and an N-terminal His-tag fusion | Marinobacter nauticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0273 | - |
ricinoleoyl-CoA, pH 7.0, 30°C | Marinobacter nauticus |
0.043 | - |
palmitoyl-CoA, pH 7.0, 30°C | Marinobacter nauticus |
0.0677 | - |
stearoyl-CoA, pH 7.0, 30°C | Marinobacter nauticus |
0.0774 | - |
icosanoyl-CoA, pH 7.0, 30°C | Marinobacter nauticus |
0.1082 | - |
oleoyl-CoA, pH 7.0, 30°C | Marinobacter nauticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
erucoyl-CoA + NADPH + H+ | erucoyl-CoA i.e. (13Z)-docos-13-enoyl-CoA | Marinobacter nauticus | (13Z)-docos-13-enal + CoA + NADP+ | further direct reduction of (13Z)-docos-13-enal into the corresponding (13Z)-docos-13-en-1-ol | ? | |
icosanoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | icosanal + CoA + NADP+ | further direct reduction of icosanal into the corresponding icosan-1-ol | ? | |
additional information | in contrast to other prokaryotes, the enzyme performs the two reduction steps from acyl-CoA to fatty alcohol in a single step which is typical for eukaryotes | Marinobacter nauticus | ? | - |
? | |
additional information | shorter acyl chains than 16 carbon are less effectively utilized and with these substrates substantial amounts of both free fatty acids and fatty aldehyde are formed | Marinobacter nauticus | ? | - |
? | |
oleoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | (9Z)-octadec-9-enal + CoA + NADP+ | further direct reduction of (9Z)-octadec-9-enal into the corresponding (9Z)-octadec-9-en-1-ol | ? | |
palmitoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | hexadecanal + CoA + NADP+ | further direct reduction of hexadecanal into the corresponding hexadecan-1-ol | ? | |
palmitoyl-[acyl-carrier protein] + NADPH + H+ | - |
Marinobacter nauticus | hexadecanal + [acyl-carrier protein] + NADP+ | further direct reduction of hexadecanal into the corresponding hexadecan-1-ol | ? | |
ricinoleoyl-CoA + NADPH + H+ | ricinoleoyl-CoA i.e. (9Z,12R)-12-hydroxyoctadec-9-enoyl-CoA | Marinobacter nauticus | (9Z,12R)-12-hydroxyoctadec-9-enal + CoA + NADP+ | further direct reduction of (9Z,12R)-12-hydroxyoctadec-9-enal into the corresponding (9Z,12R)-octadec-9-ene-1,12-diol | ? | |
stearoyl-CoA + NADPH + H+ | - |
Marinobacter nauticus | octadecanal + CoA + NADP+ | further direct reduction of octadecanal into the corresponding octadecan-1-ol | ? |