Cloned (Comment) | Organism |
---|---|
PdcG with His6 tag inserted into expression vectors pET30a and expressed in Escherichia coli BL21 (DE3) cells | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 83.2% activity compared to untreated control | Pseudomonas sp. | |
SDS | 5 mM, 81.6% activity compared to untreated control | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 5 mM chloride salt, 123.3% activity compared to untreated control | Pseudomonas sp. | |
Cd2+ | 5 mM sulfate salt, 58.9% activity compared to untreated control | Pseudomonas sp. | |
Co2+ | 5 mM chloride salt, 147.1% activity compared to untreated control | Pseudomonas sp. | |
Cu2+ | 5 mM chloride salt, 110.2% activity compared to untreated control | Pseudomonas sp. | |
Fe2+ | 5 mM chloride salt, 39.5% activity compared to untreated control | Pseudomonas sp. | |
Fe3+ | 5 mM chloride salt, 187.4% activity compared to untreated control | Pseudomonas sp. | |
K+ | 5 mM chloride salt, 95.7% activity compared to untreated control | Pseudomonas sp. | |
Mg2+ | 5 mM chloride salt, 138.1% activity compared to untreated control | Pseudomonas sp. | |
Mn2+ | 5 mM chloride salt, 186.1% activity compared to untreated control | Pseudomonas sp. | |
Na+ | 5 mM chloride salt, 88.2% activity compared to untreated control | Pseudomonas sp. | |
Ni2+ | 5 mM chloride salt, 183.9% activity compared to untreated control | Pseudomonas sp. | |
Zn2+ | 5 mM chloride salt, 145.9% activity compared to untreated control | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
recombinant His6-PdcG by SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
isolated from methyl parathion polluted activated sludge | - |
Pseudomonas sp. 1-7 | - |
isolated from methyl parathion polluted activated sludge | - |
Purification (Comment) | Organism |
---|---|
His6-PdcG by Ni2+-NTA affinity chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.44 | - |
purified recombinant His6-PdcG enzyme, pH 8.0 at 50°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxymuconic semialdehyde + NAD+ + H2O | - |
Pseudomonas sp. | maleylacetate + NADH + 2 H+ | - |
? | |
4-hydroxymuconic semialdehyde + NAD+ + H2O | - |
Pseudomonas sp. 1-7 | maleylacetate + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-HS dehydrogenase | - |
Pseudomonas sp. |
4-hydroxymuconic semialdehyde dehydrogenase | - |
Pseudomonas sp. |
PdcG | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | - |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme retains 65% activity after 20 min at 60°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | 18% activity after 30 min at pH 3.0, and 75% activity after 30 min at pH 10.0 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme is part of the degradation pathway of 4-nitrophenol which is a priority environmental pollutant | Pseudomonas sp. |