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Literature summary for 1.2.1.61 extracted from

  • Zhang, S.; Sun, W.; Xu, L.; Zheng, X.; Chu, X.; Tian, J.; Wu, N.; Fan, Y.
    Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in Pseudomonas sp. 1-7 (2012), BMC Microbiol., 12, 27.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.61

Cloned(Commentary)

Cloned (Comment) Organism
PdcG with His6 tag inserted into expression vectors pET30a and expressed in Escherichia coli BL21 (DE3) cells Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 83.2% activity compared to untreated control Pseudomonas sp.
SDS 5 mM, 81.6% activity compared to untreated control Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 5 mM chloride salt, 123.3% activity compared to untreated control Pseudomonas sp.
Cd2+ 5 mM sulfate salt, 58.9% activity compared to untreated control Pseudomonas sp.
Co2+ 5 mM chloride salt, 147.1% activity compared to untreated control Pseudomonas sp.
Cu2+ 5 mM chloride salt, 110.2% activity compared to untreated control Pseudomonas sp.
Fe2+ 5 mM chloride salt, 39.5% activity compared to untreated control Pseudomonas sp.
Fe3+ 5 mM chloride salt, 187.4% activity compared to untreated control Pseudomonas sp.
K+ 5 mM chloride salt, 95.7% activity compared to untreated control Pseudomonas sp.
Mg2+ 5 mM chloride salt, 138.1% activity compared to untreated control Pseudomonas sp.
Mn2+ 5 mM chloride salt, 186.1% activity compared to untreated control Pseudomonas sp.
Na+ 5 mM chloride salt, 88.2% activity compared to untreated control Pseudomonas sp.
Ni2+ 5 mM chloride salt, 183.9% activity compared to untreated control Pseudomonas sp.
Zn2+ 5 mM chloride salt, 145.9% activity compared to untreated control Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 recombinant His6-PdcG by SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 isolated from methyl parathion polluted activated sludge
-
Pseudomonas sp. 1-7
-
 isolated from methyl parathion polluted activated sludge
-

Purification (Commentary)

Purification (Comment) Organism
His6-PdcG by Ni2+-NTA affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.44
-
 purified recombinant His6-PdcG enzyme, pH 8.0 at 50°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxymuconic semialdehyde + NAD+ + H2O
-
 Pseudomonas sp. maleylacetate + NADH + 2 H+
-
 ?
4-hydroxymuconic semialdehyde + NAD+ + H2O
-
 Pseudomonas sp. 1-7 maleylacetate + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
4-HS dehydrogenase
-
 Pseudomonas sp.
4-hydroxymuconic semialdehyde dehydrogenase
-
 Pseudomonas sp.
PdcG
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 purified enzyme retains 65% activity after 20 min at 60°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 18% activity after 30 min at pH 3.0, and 75% activity after 30 min at pH 10.0 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas sp.

General Information

General Information Comment Organism
metabolism enzyme is part of the degradation pathway of 4-nitrophenol which is a priority environmental pollutant Pseudomonas sp.