Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces sp. NL15-2K |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Streptomyces sp. NL15-2K | |
Cu2+ | 79% inhibition at 1 mM | Streptomyces sp. NL15-2K | |
additional information | not affected by Co2+, Fe2+, Mg2+, Mn2+, and Fe3+ | Streptomyces sp. NL15-2K | |
Zn2+ | 15% inhibition at 1 mM | Streptomyces sp. NL15-2K |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00847 | - |
Vanillin | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
0.566 | - |
Salicylaldehyde | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
0.647 | - |
NAD+ | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
214000 | - |
gel filtration | Streptomyces sp. NL15-2K |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. NL15-2K | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, Resource Phe column chromatography, and Mono Q column chromatography | Streptomyces sp. NL15-2K |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzaldehyde + NAD+ + H2O | 56% activity compared to vanillin | Streptomyces sp. NL15-2K | 4-hydroxybenzoate + NADH + 2 H+ | - |
? | |
anisaldehyde + NAD+ + H2O | 15% activity compared to vanillin | Streptomyces sp. NL15-2K | anisoate + NADH + 2 H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 79% activity compared to vanillin | Streptomyces sp. NL15-2K | benzoate + NADH + 2 H+ | - |
? | |
isovanillin + NAD+ + H2O | 49% activity compared to vanillin | Streptomyces sp. NL15-2K | isovanillate + NADH + 2 H+ | - |
? | |
additional information | no activity with NADP+ or syringaldehyde | Streptomyces sp. NL15-2K | ? | - |
- |
|
protocatechualdehyde + NAD+ + H2O | 91% activity compared to vanillin | Streptomyces sp. NL15-2K | protocatechuate + NADH + 2 H+ | - |
? | |
salicylaldehyde + NAD+ + H2O | 48% activity compared to vanillin | Streptomyces sp. NL15-2K | salicylate + NADH + 2 H+ | - |
? | |
vanillin + NAD+ + H2O | 100% activity | Streptomyces sp. NL15-2K | vanillate + NADH + 2 H+ | - |
? | |
veratraldehyde + NAD+ + H2O | 12% activity compared to vanillin | Streptomyces sp. NL15-2K | veratrate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 56000, SDS-PAGE | Streptomyces sp. NL15-2K |
homotetramer | 4 * 51705, calculated from amino acid sequence | Streptomyces sp. NL15-2K |
Synonyms | Comment | Organism |
---|---|---|
VDH | - |
Streptomyces sp. NL15-2K |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Streptomyces sp. NL15-2K |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | after 10 min at 20-70°C, the enzyme activity is retained up to 40°C but declines sharply above 45°C, and the enzyme solution incubated at 50°C becomes slightly clouded | Streptomyces sp. NL15-2K |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.46 | - |
Vanillin | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
14.91 | - |
Salicylaldehyde | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
23.45 | - |
NAD+ | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Streptomyces sp. NL15-2K |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | at all pH ranges tested, the enzyme loses more than 40% of its initial activity after storage at 4°C for 15 h, and the enzyme solution becomes clouded after storage at pH 5.0 or less | Streptomyces sp. NL15-2K |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptomyces sp. NL15-2K |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces sp. NL15-2K | calculated from amino acid sequence | - |
5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
Salicylaldehyde | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
36 | - |
NAD+ | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
1110 | - |
Vanillin | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K |