Application | Comment | Organism |
---|---|---|
synthesis | integration of multiple copies of malonyl-CoA reductase MCR and of phosphorylation-deficient acetyl-CoA carboxylase ACC1 genes into the genome of yeast increases 3-hydroxypropionic acid titer fivefold in comparison with single integration. Optimizing the supply of acetyl-CoA by overexpressing native pyruvate decarboxylase PDC1, aldehyde dehydrogenase ALD6, and acetyl-CoA synthase from Salmonella enterica SEacsL641P engineering the cofactor specificity of the glyceraldehyde-3-phosphate dehydrogenase to increase the intracellular production of NADPH at the expense of NADH improves 3-hydroxypropionic acid production and reduces formation of glycerol as by-product. The final strain produces 9.8 g per L 3-hydroxypropionic acid with a yield of 13% C-mol per C-mol glucose after 100 h in carbon-limited fed-batch cultivation at pH 5 | Chloroflexus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | Q6QQP7 | - |
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