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Literature summary for 1.2.1.77 extracted from

  • Bajns J., Boulanger MJ.
    Structural and biochemical characterization of a novel aldehyde dehydrogenase encoded by the benzoate oxidation pathway in Burkholderia xenovorans LB400 (2008), J. Mol. Biol., 379, 597-608.
    View publication on PubMed
Search for more literature for 1.2.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Paraburkholderia xenovorans

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method, at 18°C in 29% PEG 3350K and 100 mM bis-Tris (pH 6.0), 1.6 A crystal structure of ALDHC in complex with NADPH bound in the cofactor-binding pocket and an ordered fragment of a polyethylene glycol molecule bound in the substrate tunnel Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NADP+ in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
0.042
-
 heptaldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
0.3
-
 Valeraldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
0.501
-
 NAD+ in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
1.21
-
 propionaldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
1.66
-
 Isovaleraldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
1.9
-
 formaldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
4.15
-
 benzaldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O Paraburkholderia xenovorans the enzyme is involved in the benzoate oxidation (box) pathway 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans Q13WK4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O the enzyme is involved in the benzoate oxidation (box) pathway Paraburkholderia xenovorans 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?
3,4-didehydroadipyl-CoA semialdehyde + NAD+ + H2O
-
 Paraburkholderia xenovorans 3,4-didehydroadipyl-CoA + NADH + H+
-
 r
benzaldehyde + NADP+ + H2O
-
 Paraburkholderia xenovorans benzoate + NADPH + H+
-
 ?
formaldehyde + NADP+ + H2O
-
 Paraburkholderia xenovorans formate + NADPH + H+
-
 ?
heptaldehyde + NADP+ + H2O
-
 Paraburkholderia xenovorans heptanoate + NADPH + H+
-
 ?
isovaleraldehyde + NADP+ + H2O
-
 Paraburkholderia xenovorans isovalerate + NADPH + H+
-
 ?
additional information the native substrate (3,4-dehydroadipyl-CoA semialdehyde) is not tested as it is commercially unavailable. The enzyme is preferentially active towards linear medium-chain to long-chain aldehydes as compared to branched-chain, short-chain or aromatic aldehydes Paraburkholderia xenovorans ?
-
 ?
propionaldehyde + NADP+ + H2O
-
 Paraburkholderia xenovorans propionate + NADPH + H+
-
 ?
valeraldehyde + NADP+ + H2O
-
 Paraburkholderia xenovorans valerate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? forms a dimer in solution with a molecular mass of 110000, SDS-PAGE Paraburkholderia xenovorans

Synonyms

Synonyms Comment Organism
ALDHC
-
 Paraburkholderia xenovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.019
-
 benzaldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
0.04
-
 NADP+ in 50 mM Tris-HCl (pH 7.5), at 25°C, cosubstrate: propionaldehyde Paraburkholderia xenovorans
0.046
-
 formaldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
0.074
-
 Isovaleraldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
0.501
-
 NAD+ in 50 mM Tris-HCl (pH 7.5), at 25°C, cosubstrate: propionaldehyde Paraburkholderia xenovorans
2.32
-
 propionaldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
5.16
-
 Valeraldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans
29.9
-
 heptaldehyde in 50 mM Tris-HCl (pH 7.5), at 25°C Paraburkholderia xenovorans

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme is more active in the presence of NADP+ relative to NAD+. Crystallographic data show that cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on the enzyme Paraburkholderia xenovorans
NADP+ the enzyme is more active in the presence of NADP+ relative to NAD+. Crystallographic data show that cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on the enzyme Paraburkholderia xenovorans