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Literature summary for 1.2.1.79 extracted from
- Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z (1976), Biochim. Biophys. Acta, 429, 55-62.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 1 mM, 78% activation | Euglena gracilis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | 0.01 mM, 29% residual activity | Euglena gracilis | |
| Ca2+ | - |
Euglena gracilis | |
| Mg2+ | - |
Euglena gracilis | |
| Mn2+ | - |
Euglena gracilis | |
| N-ethylmaleimide | 0.1 mM, complete loss of activity | Euglena gracilis | |
| Ni2+ | - |
Euglena gracilis | |
| succinate semialdehyde | substrate inhibition above 1 mM | Euglena gracilis | |
| Zn2+ | 1 mM, complete inhibition | Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
NADP+ | pH 8.7, 35°C | Euglena gracilis | |
| 1.06 | - |
succinate semialdehyde | pH 8.7, 35°C | Euglena gracilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 5 mM, 10% activation | Euglena gracilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
pH 8.7, 35°C | Euglena gracilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, unstable unless 30% glycerol is added | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonate semialdehyde + NADP+ + H2O | 8.7% of the activity with succinate semialdehyde | Euglena gracilis | malonate + NADPH + 2 H+ | - |
? | |
| additional information | no substrate: glyoxylic acid, formic acid, formaldehyde, acetaldehyde, glyoxal, furfural and acrolein | Euglena gracilis | ? | - |
? | |
| succinate semialdehyde + NADP+ + H2O | - |
Euglena gracilis | succinate + NADPH + 2 H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | - |
Euglena gracilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Euglena gracilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Euglena gracilis | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Euglena gracilis | induction by growth on glutamate | up |
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